Epidermal reconstructs: a new tool to study topical and systemic photoprotective molecules
| Autor: | Mauro Picardo, Alain Taieb, Yvon Gall, Stefania Briganti, Muriel Cario-André, José Ginestar, Osamu Nikaido |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Antioxidant Ultraviolet Rays medicine.medical_treatment Biophysics Sunburn Radiation-Protective Agents Pyrimidine dimer Protein oxidation Antioxidants Superoxide dismutase Lipid oxidation Reference Values medicine Humans Vitamin E Radiology Nuclear Medicine and imaging Cells Cultured Radiation Radiological and Ultrasound Technology biology Superoxide Dismutase Chemistry Infant Newborn Catalase Circumcision Male Biochemistry Pyrimidine Dimers Photoprotection biology.protein Melanocytes Lipid Peroxidation Epidermis Sunscreening Agents |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 68:79-87 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/s1011-1344(02)00328-7 |
| Popis: | In this study, we compared the effects of sunscreens and antioxidants on reconstructed epidermis made with or without melanocytes 24 h after UVB, UVA or UVA+B irradiation. For this purpose, we studied sunburn cells and cyclobutane pyrimidine dimer formation, protein and lipid oxidation, catalase and superoxide dismutase activities and vitamin E levels. Topical sunscreens protected against direct cell death and thymine dimer formation whereas their protective effect against protein and lipid oxidation and antioxidant depletion was less marked partly due to the difficulty of spreading the cream. Antioxidant molecules protected against direct cell death and protein oxidation but not against thymine dimer formation. Since topical sunscreens and systemic antioxidant protected the skin differently, we speculate that their association might protect more efficiently against UV-induced damage. This model is relevant to study systemic molecules but is less practical, due to the technical limitations of studying topical molecules. |
| Databáze: | OpenAIRE |
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