Neurons and astroglia express distinct subsets of Na,K-ATPase alpha and beta subunits
| Autor: | Andrew W. Shyjan, Robert Levenson, Richard S. Cameron, Laura E. Klein, Pasko Rakic |
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| Rok vydání: | 1994 |
| Předmět: |
Gene isoform
Protein subunit Immunoblotting Biology Cellular and Molecular Neuroscience medicine Animals Molecular Biology In Situ Hybridization G alpha subunit Neurons Brain Granule cell Molecular biology Macaca mulatta Rats Isoenzymes medicine.anatomical_structure Microscopy Fluorescence Astrocytes biology.protein Neuroglia Neuron Sodium-Potassium-Exchanging ATPase ATP synthase alpha/beta subunits Biomarkers Astrocyte |
| Zdroj: | Brain research. Molecular brain research. 21(3-4) |
| ISSN: | 0169-328X |
| Popis: | We have analyzed the expression pattern of Na,K-ATPase alpha and beta subunit isoforms within the rodent and primate central nervous system. Membrane fractions prepared from rat cerebral cortical type-1 astrocytes and rat cerebellar granule and hippocampal neurons were characterized by immunoblot analyses using a panel of alpha and beta subunit isoform-specific antisera. Each cell type was found to express the alpha 1 isoform but showed differences in the expression of other subunits. Cortical astrocytes displayed alpha 2 and beta 2 subunits, whereas cerebellar granule neurons showed expression of alpha 3 and beta 1 subunits. All three alpha subunit isotypes were detected in hippocampal neurons. A survey of the immunofluorescent staining pattern of the alpha 3 subunit in rat and monkey brain confirmed that expression of this Na,K-ATPase alpha subunit isoform was restricted exclusively to neurons. These results suggest that both neurons and astrocytes express multiple, yet distinct, Na,K-ATPase isoenzymes. The identification of cell types expressing limited combinations of alpha and beta subunits should provide a framework for understanding the physiological significance of Na,K-ATPase isoenzyme diversity and may provide useful tools for the analysis of cell lineage in the mammalian central nervous system. |
| Databáze: | OpenAIRE |
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