Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains
| Autor: | V. Pirson, Frédéric Goffaux, Jacques Mainil, H. J. Ball, Ioan Mihai Szalo, Denis Pierard |
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| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Operon Escherichia coli O157 medicine.disease_cause Microbiology parasitic diseases Escherichia coli medicine Animals Humans Molecular Biology Gene Intimin Adhesins Escherichia coli biology Reverse Transcriptase Polymerase Chain Reaction General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Intestinal epithelium Virology Enterobacteriaceae Bacterial adhesin bacteria Cattle Bacteria |
| Zdroj: | Research in Microbiology. 153:653-658 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/s0923-2508(02)01379-7 |
| Popis: | Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC) infections are characterised by the formation of attaching and effacing lesions on intestinal epithelial cells. The first step of EPEC and EHEC pathogenesis involves the initial adherence of the bacterium to the intestinal epithelium. A collection of bovine EPEC and EHEC strains belonging to different serogroups was tested by colony blot hybridization with gene probes for putative adhesins (BFPA, LPFA, IHA, LIFA) of human EPEC and EHEC, and also for fimbrial and afimbrial adhesins (AFA8, F17, Cs31A) of bovine necrotoxigenic E. coli (NTEC). In the bovine EPEC and EHEC strains tested, sequences homologous to lifA, ihA, and lpfA genes were detected, sometimes in association with particular serogroups. Bovine 026 EPEC also possessed a sequence homologous to a gene of the c/p operon, coding for the CS31A adhesin, associated with bovine NTEC. Overall results showed that different genes encoding for putative adhesins of human EHEC strains are present in bovine EPEC and EHEC strains, but not one of them is present in all strains. |
| Databáze: | OpenAIRE |
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