Label-Free Electrochemical Detection of the Specific Oligonucleotide Sequence of Dengue Virus Type 1 on Pencil Graphite Electrodes
| Autor: | Elaine V.M. Souza, Edna Natividade, Gustavo Nascimento, Danyelly Bruneska Gondim Martins, Nataly Santana, Danielly Ferreira, Manoel Lima, José L. Lima-Filho |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Working electrode
Guanine Time Factors Analytical chemistry Oligonucleotides Biosensing Techniques lcsh:Chemical technology Biochemistry Article Analytical Chemistry dengue virus nucleic acid biosensor guanine oxidation Nucleic Acids Electrochemistry Humans lcsh:TP1-1185 Graphite Electrical and Electronic Engineering Instrumentation Electrodes Detection limit Chemistry Oligonucleotide Hybridization probe Computational Biology Nucleic Acid Hybridization Hydrogen-Ion Concentration Atomic and Molecular Physics and Optics Electrode DNA Viral Differential pulse voltammetry Adsorption DNA Probes Biosensor Nuclear chemistry |
| Zdroj: | Sensors; Volume 11; Issue 6; Pages: 5616-5629 Sensors (Basel, Switzerland) Sensors, Vol 11, Iss 6, Pp 5616-5629 (2011) |
| ISSN: | 1424-8220 |
| DOI: | 10.3390/s110605616 |
| Popis: | A biosensor that relies on the adsorption immobilization of the 18-mer single-stranded nucleic acid related to dengue virus gene 1 on activated pencil graphite was developed. Hybridization between the probe and its complementary oligonucleotides (the target) was investigated by monitoring guanine oxidation by differential pulse voltammetry (DPV). The pencil graphite electrode was made of ordinary pencil lead (type 4B). The polished surface of the working electrode was activated by applying a potential of 1.8 V for 5 min. Afterward, the dengue oligonucleotides probe was immobilized on the activated electrode by applying 0.5 V to the electrode in 0.5 M acetate buffer (pH 5.0) for 5 min. The hybridization process was carried out by incubating at the annealing temperature of the oligonucleotides. A time of five minutes and concentration of 1 μM were found to be the optimal conditions for probe immobilization. The electrochemical detection of annealing between the DNA probe (TS-1P) immobilized on the modified electrode, and the target (TS-1T) was achieved. The target could be quantified in a range from 1 to 40 nM with good linearity and a detection limit of 0.92 nM. The specificity of the electrochemical biosensor was tested using non-complementary sequences of dengue virus 2 and 3. |
| Databáze: | OpenAIRE |
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