Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors
Autor: | Michael H. Luethy, Larry A. Gilbertson, Qiang Zhang, Darren H Birr, Mark W Snyder, Kathleen P. Malloy, Thomas H. Adams, Shihshieh Huang, Emily K Reisenbigler |
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Rok vydání: | 2004 |
Předmět: |
Genetics
Recombination Genetic DNA Plant Agrobacterium fungi food and beverages Biology biology.organism_classification Plants Genetically Modified Marker gene Zea mays Transformation (genetics) Plasmid Transformation Genetic Cotransformation Agrobacterium tumefaciens Genes Reporter Animal Science and Zoology Agronomy and Crop Science Gene T-DNA Binary system Selectable marker Biotechnology Plasmids |
Zdroj: | Transgenic research. 13(5) |
ISSN: | 0962-8819 |
Popis: | By introducing additional T-DNA borders into a binary plasmid used in Agrobacterium-mediated plant transformation, previous studies have demonstrated that the marker gene and the gene of interest (GOI) can be carried by independent T-strands, which sometimes integrate in unlinked loci in the plant genome. This allows the recovery of marker-free transgenic plants through genetic segregation in the next generation. In this study, we have found that by repositioning the selectable marker gene in the backbone and leaving only the GOI in the T-DNA region, a regular two-border binary plasmid was able to generate marker-free transgenic maize plants more efficiently than a conventional single binary plasmid with multiple T-DNA borders. These results also provide evidence that both the right and left borders can initiate and terminate T-strands. Such non-canonical initiation and termination of T-strands may be the basis for the elevated frequencies of cotransformation and unlinked insertions. |
Databáze: | OpenAIRE |
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