Noise analysis of cytosolic calcium image data
| Autor: | Angelo Demuro, Ian Parker, Divya Swaminathan, George D. Dickinson |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Biochemistry & Molecular Biology Physiology Xenopus Medical Physiology Gating Noise analysis Article Fluorescence Image (mathematics) Cell Line Imaging 03 medical and health sciences 0302 clinical medicine Imaging Three-Dimensional Cytosol Rare Diseases Ca2+ imaging Cell Line Tumor Catalytic Domain Inositol 1 4 5-Trisphosphate Receptors Animals Humans Ca2+ noise Molecular Biology 5-Trisphosphate Receptors Physics Total internal reflection fluorescence microscope Tumor Ca(2+) puffs Spectral density Cell Biology Ca2+ puffs Inositol 1 Ca(2+) imaging Electrophysiology Noise Kinetics 030104 developmental biology Three-Dimensional Ca(2+) noise Calcium Calcium Channels Biochemistry and Cell Biology Biological system 030217 neurology & neurosurgery Cytosolic calcium |
| Zdroj: | Cell Calcium |
| Popis: | Cellular Ca(2+) signals are often constrained to cytosolic micro- or nano-domains where stochastic openings of Ca(2+) channels cause large fluctuations in local Ca(2+) concentration (Ca(2+) ‘noise’). With the advent of TIRF microscopy to image the fluorescence of Ca(2+)-sensitive probes from attoliter volumes it has become possible to directly monitor these signals, which closely track the gating of plasmalemmal and ER Ca(2+) permeable channels. Nevertheless, it is likely that many physiologically important Ca(2+) signals are too small to resolve as discrete events in fluorescence recordings. By analogy with noise analysis of electrophysiological data, we explore here the use of statistical approaches to detect and analyze such Ca(2+) noise in images obtained using Ca(2+)-sensitive indicator dyes. We describe two techniques - power spectrum analysis and spatio-temporal correlation - and demonstrate that both effectively identify discrete, spatially localized calcium release events (Ca(2+) puffs). Moreover, we show they are able to detect localized noise fluctuations in a case where discrete events cannot directly be resolved. |
| Databáze: | OpenAIRE |
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