Effects of hemoglobin C, D, E and S traits on measurements of hemoglobin A1c by twelve methods
| Autor: | Ross J. Molinaro, Carla Siebelder, Steven Hanson, Cas Weykamp, Randie R. Little, Curt L. Rohlfing, Trefor Higgins, Paul M. Yip |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Thalassemia Clinical Biochemistry 030204 cardiovascular system & hematology Boronate affinity Biochemistry Chromatography Affinity Article 03 medical and health sciences Deming regression Hemoglobins 0302 clinical medicine Internal medicine Linear regression medicine Humans Chromatography High Pressure Liquid Glycated Hemoglobin business.industry Biochemistry (medical) Hemoglobin variants Electrophoresis Capillary General Medicine medicine.disease Chromatography Ion Exchange Hemoglobin C Hemoglobin A Endocrinology 030220 oncology & carcinogenesis Immunology Hemoglobin business |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 455 |
| ISSN: | 1873-3492 |
| Popis: | Background Hemoglobin C, D Punjab, E or S trait can interfere with hemoglobin A1c (HbA1c) results. We assessed whether they affect results obtained with 12 current assay methods. Methods Hemoglobin AA (HbAA), HbAC, HbAD Punjab, HbAE and HbAS samples were analyzed on one enzymatic, nine ion-exchange HPLC and two Capillary Electrophoresis methods. Trinity ultra2 boronate affinity HPLC was the comparative method. An overall test of coincidence of least-squared linear regression lines was performed to determine if HbA1c results were statistically significantly different from those of HbAA samples. Clinically significant interference was defined as > 7% difference from HbAA at 6 or 9% HbA1c compared to ultra2 using Deming regression. Results All methods showed statistically significant effects for one or more variants. Clinically significant effects were observed for the Tosoh G8 variant mode and GX (all variants), GX V1.22 (all but HbAE) and G11 variant mode (HbAC). All other methods (Abbott Architect c Enzymatic, Bio-Rad D-100, Variant II NU and Variant II Turbo 2.0, Menarini HA-8180T thalassemia mode and HA-8180V variant mode, Sebia Capillarys 2 and Capillarys 3) showed no clinically significant differences. Conclusions Several methods showed clinically significant interference with HbA1c results from one or more variants which could adversely affect patient care. |
| Databáze: | OpenAIRE |
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