Microfluidic channel integrated with a lattice lightsheet microscopic system for continuous cell imaging
Autor: | Bi-Chang Chen, Chieh-Han Lu, Han Yun Hsieh, Sheng Fang Tsai, Yen-Ting Liu, Cheng Hsuan Wu, Yu Jui Fan, Chia Ming Lee, Shu-Wei Chang |
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Rok vydání: | 2021 |
Předmět: |
Materials science
Microfluidics Biomedical Engineering Bioengineering 02 engineering and technology Biochemistry Light scattering 03 medical and health sciences Imaging Three-Dimensional Optics Microfluidic channel Lattice (order) Microscopy Perpendicular Thin film 030304 developmental biology 0303 health sciences business.industry General Chemistry Image plane 021001 nanoscience & nanotechnology Refractometry 0210 nano-technology business |
Zdroj: | Lab on a Chip. 21:344-354 |
ISSN: | 1473-0189 1473-0197 |
Popis: | In this study, a continuous cell-imaging system with subcellular resolution was developed by integrating a microfluidic platform with lattice lightsheet microscopy (LLSM). To reduce aberrations of the lightsheet propagating into the device, a microfluidic channel sealed with a water refractive index-matched thin film was fabricated. When the lightsheet emerged from the water-immersed objectives and penetrated through the water refractive-matched thin film into the microfluidic channel at an incident angle, less light scattering and fewer aberrations were found. Suspended cells flowed across the lattice lightsheet, and an imaging system with the image plane perpendicular to the lightsheet was used to sequentially acquire cell images. By applying a thinner lattice lightsheet, higher-resolution, higher-contrast images were obtained. Furthermore, three-dimensional cell images could be achieved by reconstructing sequential two-dimensional cell images. |
Databáze: | OpenAIRE |
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