Mouse IgG3 binding to macrophage-like cells is prevented by deglycosylation of the antibody or by Accutase treatment of the cells
| Autor: | Alicja Karabasz, Tomasz Klaus, Monika Bzowska, Maja Sochalska, Joanna Bereta, Maria Czarnek |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Integrins Glycosylation Science Phagocytosis Integrin Immunology Phase-contrast microscopy chemical and pharmacologic phenomena complex mixtures Biochemistry Article Flow cytometry 03 medical and health sciences Mice 0302 clinical medicine Immune system parasitic diseases medicine Macrophage Animals Collagenases Receptor Monocytes and macrophages Antibody isolation and purification Multidisciplinary medicine.diagnostic_test biology Chemistry Macrophages Antimicrobial responses Flow Cytometry Cellular immunity Cell biology Humoral immunity 030104 developmental biology Cell culture Immunoglobulin G biology.protein Medicine Antibody 030215 immunology Peptide Hydrolases Protein Binding |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
| ISSN: | 2045-2322 |
| Popis: | The binding of mouse IgG3 to Fcγ receptors (FcγR) and the existence of a mouse IgG3-specific receptor have been discussed for 40 years. Recently, integrin beta-1 (ITGB1) was proposed to be a part of an IgG3 receptor involved in the phagocytosis of IgG3-coated pathogens. We investigated the interaction of mouse IgG3 with macrophage-like J774A.1 and P388D1 cells. The existence of an IgG3-specific receptor was verified using flow cytometry and a rosetting assay, in which erythrocytes clustered around the macrophage-like cells coated with an erythrocyte-specific IgG3. Our findings confirmed that receptors binding antigen-free IgG3 are present on J774A.1 and P388D1 cells. We demonstrated for the first time that the removal of N-glycans from IgG3 completely abolished its binding to the cells. Moreover, we discovered that the cells treated with Accutase did not bind IgG3, indicating that IgG3-specific receptors are substrates of this enzyme. The results of antibody-mediated blocking of putative IgG3 receptors suggested that apart from previously proposed ITGB1, FcγRII, FcγRIII, also additional, still unknown, receptor is involved in IgG3 binding. These findings indicate that there is a complex network of glycan-dependent interactions between mouse IgG3 and the surface of effector immune cells. |
| Databáze: | OpenAIRE |
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