Gold nanorods–based multicolor immunosensor for visual detection of enterovirus 71 infection
| Autor: | Yan-Song Li, Shi-Ying Lu, Zhaozhao Si, Shujuan Han, Hong-Lin Ren, Yuying Yang, Xiongyan Liang, Yunqing Liu, Pan Hu, Baoling Zhao, Yu Zhou, Fang Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Chromatography
biology Igm antibody Chemistry Biosensing Techniques 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Horseradish peroxidase Enterovirus A Human 0104 chemical sciences Analytical Chemistry Visual detection biology.protein Enterovirus 71 Humans Nanorod Gold Surface plasmon resonance 0210 nano-technology |
| Zdroj: | Microchimica Acta. 187 |
| ISSN: | 1436-5073 0026-3672 |
| DOI: | 10.1007/s00604-020-04534-x |
| Popis: | Based on the etching of gold nanorods (GNRs) and enzyme-linked immunosorbent assay (ELISA), a multicolor immunosensor for visual detection of enterovirus 71 infection is proposed. Once the immunocomplex is formed, the horseradish peroxidase bound to the ELISA plate oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) into TMB2+ in the presence of hydrogen peroxide. Subsequently, TMB2+ quantitatively etches GNRs to the short GNRs, leading to a blue shift of longitudinal localized surface plasmon resonance and corresponding color responses. This change is used to develop two types of cut-off standards, which respond to the human anti-enterovirus at a concentration of 71 IgM antibody. The method has been validated with clinical serum samples and showed high sensitivity and specificity . This visual immunosensor has an important application value for point-of-care detection of EV71, especially in areas lacking detection equipment. |
| Databáze: | OpenAIRE |
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