Cytotoxic Activity of Christia vespertilionis Root and Leaf Extracts and Fractions against Breast Cancer Cell Lines

Autor: Latifah Saiful Yazan, Pei Cee Lim, Nurulaidah Esa, Nur Kartinee Kassim, Joanna Jinling Lee, Che Azurahanim Che Abdullah, Dai Chuan Tan
Jazyk: angličtina
Rok vydání: 2020
Předmět:
antioxidant
Antioxidant
DPPH
MDA-MB-231
medicine.medical_treatment
Phytochemicals
Pharmaceutical Science
Apoptosis
Decoction
Plant Roots
Antioxidants
Analytical Chemistry
chemistry.chemical_compound
0302 clinical medicine
Christia vespertilionis
Drug Discovery
Tumor Cells
Cultured
Cytotoxicity
0303 health sciences
Traditional medicine
alternative medicine
Chemistry
Fabaceae
Free Radical Scavengers
daun rerama
Phytochemical
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Molecular Medicine
cytotoxicity
Female
Breast Neoplasms
anticancer
Article
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
medicine
Maceration (wine)
Humans
MTT assay
Physical and Theoretical Chemistry
Cell Proliferation
030304 developmental biology
leaf
Plant Extracts
Organic Chemistry
root
Antineoplastic Agents
Phytogenic
Plant Leaves
MCF-7
Zdroj: Molecules, Vol 25, Iss 2610, p 2610 (2020)
Molecules
Volume 25
Issue 11
ISSN: 1420-3049
Popis: Christia vespertilionis, commonly known as &lsquo
Daun Rerama&rsquo
has recently garnered attention from numerous sources in Malaysia as an alternative treatment. Its herbal decoction was believed to show anti-inflammatory and anti-cancer effects. The present study investigated the cytotoxicity of the extract of root and leaf of C. vespertilionis. The plant parts were successively extracted using the solvent maceration method. The most active extract was further fractionated to afford F1&ndash
F8. The cytotoxic effects were determined using MTT assay against human breast carcinoma cell lines (MCF-7 and MDA-MB-231). The total phenolic content (TPC) of the extracts were determined. The antioxidant properties of the extract were also studied using DPPH and &beta
carotene bleaching assays. The ethyl acetate root extract demonstrated selective cytotoxicity especially against MDA-MB-231 with the highest TPC and antioxidant properties compared to others (p <
0.05). The TPC and antioxidant results suggest the contribution of phenolic compounds toward its antioxidant strength leading to significant cytotoxicity. F3 showed potent cytotoxic effects while F4 showed better antioxidative strength compared to others (p <
0.05). Qualitative phytochemical screening of the most active fraction, F3, suggested the presence of flavonoids, coumarins and quinones to be responsible toward the cytotoxicity. The study showed the root extracts of C. vespertilionis to possess notable anti-breast cancer effects.
Databáze: OpenAIRE
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