Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation
| Autor: | Denise Gusmão de Oliveira, Vinícius Carvalho Porto, Ana Paula Chappuis Chocano, Jefferson Ricardo Pereira, Flora Freitas Fernandes Távora, Karin Hermana Neppelenbroek, Ricardo Almeida |
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| Rok vydání: | 2019 |
| Předmět: |
Denture Bases
Surface Properties PLACA BACTERIANA Acrylic Resins engineering.material confocal microscopy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Coating Candida albicans Cyanoacrylates General Dentistry Acrylic resin biology Chemistry Sealant Biofilm 030206 dentistry Sterilization (microbiology) biology.organism_classification Corpus albicans Ethyl cyanoacrylate Biofilms cyanoacrylates 030220 oncology & carcinogenesis visual_art denture liners biofilm formation engineering visual_art.visual_art_medium candida albicans Brazil Nuclear chemistry |
| Zdroj: | Brazilian Dental Journal, Volume: 30, Issue: 3, Pages: 266-271, Published: 03 JUN 2019 Brazilian Dental Journal v.30 n.3 2019 Brazilian Dental Journal Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1806-4760 0103-6440 |
| Popis: | The aim of this study was to verify whether modifications made in a hard chairside reline resin by an ethyl-cyanoacrylate adhesive, ECA (Super Bonder®, Loctite, Itapevi, SP, Brazil) would be able to inhibit or reduce Candida albicans biofilm formation on its surface, comparing to a commercial surface sealant (BisCover®, Bisco, Schaumburg, USA). Reline resin specimens were fabricated and randomly divided into 6 groups (n=8): CG (control group), no surface treatment; ECA1, ECA coating on the surface before sterilization; ECA2, ECA coating after sterilization; ECA3, ECA incorporated in the resin bulk; DPE1, BisCover® coating before sterilization; DPE2, BisCover® coating after sterilization. Specimens were inoculated with C. albicans SC5314 (1x107 cells/mL) and incubated for 24 h. Then, the biofilm were stained with LIVE/DEAD® BaclightTM L7007 Kit and analyzed by Confocal Laser Scanning Microscopy. The images were evaluated by bioImageL® v.2.0 software and total biovolume (µm3), viable cells (%), and covered area (%) were calculated. Data were statistically analyzed by Kruskal-Wallis and Dunn tests (p |
| Databáze: | OpenAIRE |
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