Molecular regionalization of the developing amphioxus neural tube challenges major partitions of the vertebrate brain
| Autor: | Manuel Irimia, Ildiko M. L. Somorjai, Ignacio Maeso, Laura Lopez-Blanch, Beatriz Albuixech-Crespo, Jordi Garcia-Fernàndez, Juan Antonio Moreno-Bravo, Eduardo Puelles, José Luis Ferran, Luis Puelles, Demian Burguera, Luisa Sánchez-Arrones, Paola Bovolenta, Juan Pascual-Anaya |
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| Přispěvatelé: | Marine Alliance for Science and Technology for Scotland, European Commission, Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, European Research Council, Fundación Séneca, Gobierno de la Región de Murcia, University of St Andrews. School of Biology, University of St Andrews. Marine Alliance for Science & Technology Scotland, University of St Andrews. Biomedical Sciences Research Complex, University of St Andrews. Scottish Oceans Institute |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
Central Nervous System 0301 basic medicine Embryology Embryo Nonmammalian Molecular biology QH301 Biology Nervous System Midbrain Neural tube 0302 clinical medicine Thalamus Medicine and Health Sciences Biology (General) Cervell Zebrafish In Situ Hybridization Fluorescence R2C Lancelets Mice Knockout biology General Neuroscience Gene Expression Regulation Developmental Vertebrate Brain Anatomy Biological Evolution medicine.anatomical_structure Vertebrates BDC General Agricultural and Biological Sciences Brainstem Notochords Neural plate In situ hybridization Research Article animal structures QH301-705.5 Central nervous system Molecular Probe Techniques Research and Analysis Methods Models Biological General Biochemistry Genetics and Molecular Biology QH301 03 medical and health sciences biology.animal Genetics medicine Animals Molecular Biology Techniques Pretectal area Molecular Biology Biologia molecular Body patterning Amphioxus Models Genetic General Immunology and Microbiology Organisms Biology and Life Sciences DAS biology.organism_classification Expressió gènica Probe Hybridization 030104 developmental biology Evolutionary biology Tub neural Chick embryo Gene expression 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona PLOS Biology Digital.CSIC. Repositorio Institucional del CSIC instname Recercat. Dipósit de la Recerca de Catalunya PLoS Biology PLoS Biology, Vol 15, Iss 4, p e2001573 (2017) |
| Popis: | et al. All vertebrate brains develop following a common Bauplan defined by anteroposterior (AP) and dorsoventral (DV) subdivisions, characterized by largely conserved differential expression of gene markers. However, it is still unclear how this Bauplan originated during evolution. We studied the relative expression of 48 genes with key roles in vertebrate neural patterning in a representative amphioxus embryonic stage. Unlike nonchordates, amphioxus develops its central nervous system (CNS) from a neural plate that is homologous to that of vertebrates, allowing direct topological comparisons. The resulting genoarchitectonic model revealed that the amphioxus incipient neural tube is unexpectedly complex, consisting of several AP and DV molecular partitions. Strikingly, comparison with vertebrates indicates that the vertebrate thalamus, pretectum, and midbrain domains jointly correspond to a single amphioxus region, which we termed Di-Mesencephalic primordium (DiMes). This suggests that these domains have a common developmental and evolutionary origin, as supported by functional experiments manipulating secondary organizers in zebrafish and mice. Spanish Ministry of Economy and Competitiveness and European FEDER funds (grant number BFU2014-57516-P). To Luis Puelles and Jose Luis Ferran. European Research Council (grant number ERC-StG-LS2-637591). To Manuel Irimia. Spanish Ministry of Economy and Competitiveness (grant number SEV-2012-0208). Centro de Excelencia Severo Ochoa (to CRG, Manuel Irimia). Spanish Ministry of Economy and Competitiveness (grant number BFU2014-58908-P). To Jordi GarciaFernadez. Seneca Foundation, Comunidad de Murcia (grant number 19904/ GERM/15). To Luis Puelles. Generalitat de Catalunya (grant number). ICREA Academia Prize to Jordi GarciaFernandez. Spanish Ministry of Economy and Competitiveness (grant number BFU2013-43213-P). To Paola Bovolenta. Spanish Ministry of Economy and Competitiveness (grant number BFU2014-55076-P). To Manuel Irimia. Including an FPI PhD fellowship to Laura Lopez-Blanch. Marine Alliance for Science and Technology Scotland (MASTS) (grant number). To Ildiko Somorjai. |
| Databáze: | OpenAIRE |
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