Asperosaponin VI Injection Enhances Orthodontic Tooth Movement in Rats
| Autor: | Jun Zhang, Xu-sheng Ren, Xuxia Wang, Dan Ma, De-Hua Zheng, Jie Bu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Molar
medicine.medical_specialty China Tooth Movement Techniques H&E stain Root Resorption Osteoclasts 030204 cardiovascular system & hematology Bone resorption Tooth Movement Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Osteoclast Osteogenesis Internal medicine medicine Alveolar Process Animals Bone Resorption Medicine Chinese Traditional Dental alveolus biology Chemistry Animal Study General Medicine Periodontium Saponins Rats medicine.anatomical_structure Endocrinology RANKL 030220 oncology & carcinogenesis Osteocalcin biology.protein Female Bone Remodeling |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND This study was performed to investigate the effect of local injection of asperosaponin VI (ASA VI) on the orthodontic tooth movement in rats. MATERIAL AND METHODS A total of 64 healthy female Sprague-Dawley rats were selected and divided into 2 groups randomly: the ASA VI group and the control group. For the ASA VI group, 10 mg/kg ASA VI solution was injected into buccal submucoperiosteal of bilaterally first maxillary molars, and the same volume of normal saline was given to the control group. The orthodontic force was applied to the maxillary first molars. All rats were sacrificed on days 3, 7, or 14. Tooth movement effects on the periodontium were analyzed through hematoxylin and eosin (H&E) staining, tartrate-resistant acid phosphatase (TRAP) staining and immunohistochemistry analysis. Tooth movement measurements and alveolar bone volumetric changes were analyzed using a micro-computed tomography (CT) scan. Molecular changes were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. RESULTS The ASA VI group presented with a significant increase of tooth movement, osteoclast number, and the expression of osteoclast differentiation factor (ODF) compared with the control group. ASA VI also induced a significant decrease in bone volume and density and an increase in trabecular spacing and RANKL (receptor activator of nuclear factor kappa-B ligand) expression at the compression side. Furthermore, ASA VI stimulated bone formation on the tension side by enhancing OCN (osteocalcin) expression and RUNX2 (runt-related transcription factor 2) expression, increasing bone volume and density and decreasing in trabecular spacing. CONCLUSIONS Injection of ASA VI may accelerate tooth movement via increasing the activity of osteoclasts, stimulating bone resorption at the compression side. Furthermore, ASA VI has a positive effect on bone formation at the tension side. |
| Databáze: | OpenAIRE |
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