Cross-adaptation to cadmium stress inPlantago ovataby pre-exposure to low dose of gamma rays: Effects on metallothionein and metal content

Autor:	Sarmistha Sen Raychaudhuri, Anindita Chakraborty, Shonima Talapatra, Pratik Talukder, Nirmalya Ghoshal, Mandar Sengupta, Suman Kumar Ray
Rok vydání:	2015
Předmět:	DNA damage chemistry.chemical_element Zinc Radiation Dosage Plantago ovata Stress Physiological Metallothionein Radiology Nuclear Medicine and imaging Plantago Cadmium Dose-Response Relationship Drug Radiological and Ultrasound Technology biology Radiochemistry food and beverages biology.organism_classification Adaptation Physiological Molecular biology Real-time polymerase chain reaction chemistry Gamma Rays Metals Seedlings Germination Shoot
Zdroj:	International Journal of Radiation Biology. 91:611-623
ISSN:	1362-3095 0955-3002
Popis:	To investigate the effects of gamma pre-exposure on cadmium accumulation in Plantago ovata seedlings. Metallothionein (MT) localization was also studied following Cadmium (Cd) treatment in P. ovata.DNA damage was determined by alkaline comet assay. MT gene and protein expression were studied by real-time polymerase chain reaction and flow cytometry, respectively, in root and shoot tissues. Metal accumulation (Cd, zinc [Zn], iron [Fe]) was evaluated by Atomic Absorption Spectroscopy.Cd treatment decreased seed germination rate, biomass and free radical scavenging activity and increased DNA damage in a dose-dependent manner. When P. ovata seeds were pre- exposed to 5 Gy gamma dose (prior to Cd treatment) seed germination rate, biomass and free radical scavenging activity increased significantly. MT genes (PoMT1, PoMT2 and PoMT3) and MT protein expression enhanced when 5 Gy gamma-irradiated seeds were grown in Cd containing medium and Cd accumulation also increased in a dose-dependent manner.Higher Cd accumulation in P. ovata seedlings may be attributed to the upregulation of PoMT genes in gamma pretreated seedlings. Localization of metallothionein in cytosol and nucleus indicated its positive role against Cd-mediated cytotoxic and genotoxic effects.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0cf7b7433f64de336ca7cda8b58212cb https://doi.org/10.3109/09553002.2015.1047984 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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