The Myb-domain protein ULTRAPETALA1 INTERACTING FACTOR 1 controls floral meristem activities in Arabidopsis

Autor: Irene López-Vidriero, José Manuel Franco-Zorrilla, François Parcy, Robert Blanvillain, Renaud Dumas, Emmanuel Thévenon, Patrice Morel, Cristel C. Carles, Christophe Trehin, Fanny Moreau
Přispěvatelé: Carles, Christel, Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Department of Plant Molecular Genetics, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Científicas [Spain] (CSIC), Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), ANR-10-JCJC-1206,ChromFlow,Dynamique de l'activation de la chromatine au cours de la morphogenèse florale(2010), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Rhone-Alpes county (Allocation Doctorale de Recherche, Cluster 7) [12-01293101], Centre National de la Recherche Scientifique (CNRS-Higher Education Chair) [0428-64], University of Grenoble-Alpes (Chaire Initiative Universitaire Alpes, UGA-UJF), French National Agency Young Researcher Grant [ChromFlow ANR JCJC] [SVSE2-1206 01], Centro Nacional de Biotecnología [Madrid] (CNB-CSIC), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Transcription
Genetic
Arabidopsis thaliana
Protein domain
Meristem
Molecular Sequence Data
Morphogenesis
Arabidopsis
Repressor
Electrophoretic Mobility Shift Assay
Flowers
03 medical and health sciences
Gene Expression Regulation
Plant
WUSCHEL
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
MYB
Protein Interaction Domains and Motifs
Amino Acid Sequence
Molecular Biology
Gene
Transcription factor
transcription factor
Genetics
Homeodomain Proteins
Stem cell
biology
Plant Stems
Arabidopsis Proteins
Stem Cells
fungi
food and beverages
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Plant
biology.organism_classification
Protein Structure
Tertiary
Plant Leaves
030104 developmental biology
Flower
Myb transcription factor
Sequence Alignment
Developmental Biology
Transcription Factors
Zdroj: Development 7 (143), 1108-1119. (2016)
Development (Cambridge, England)
Development (Cambridge, England), Company of Biologists, 2016, 143 (7), pp.1108-19
Development (Cambridge, England), Company of Biologists, 2016, 143 (7), pp.1108-19. ⟨10.1242/dev.127365⟩
Development (Cambridge, England), 2016, 143 (7), pp.1108-19. ⟨10.1242/dev.127365⟩
ISSN: 0950-1991
1477-9129
Popis: International audience; Higher plants continuously and iteratively produce new above-ground organs in the form of leaves, stems and flowers. These organs arise from shoot apical meristems whose homeostasis depends on coordination between self-renewal of stem cells and their differentiation into organ founder cells. This coordination is stringently controlled by the central transcription factor WUSCHEL (WUS), which is both necessary and sufficient for stem cell specification inArabidopsis thaliana ULTRAPETALA1 (ULT1) was previously identified as a plant-specific, negative regulator ofWUSexpression. However, molecular mechanisms underlying this regulation remain unknown. ULT1 protein contains a SAND putative DNA-binding domain and a B-box, previously proposed as a protein interaction domain in eukaryotes. Here, we characterise a novel partner of ULT1, named ULT1 INTERACTING FACTOR 1 (UIF1), which contains a Myb domain and an EAR motif. UIF1 and ULT1 function in the same pathway for regulation of organ number in the flower. Moreover, UIF1 displays DNA-binding activity and specifically binds toWUSregulatory elements. We thus provide genetic and molecular evidence that UIF1 and ULT1 work together in floral meristem homeostasis, probably by direct repression ofWUSexpression.
Databáze: OpenAIRE
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