Anti-Interleukin-1 Beta/Tumor Necrosis Factor-Alpha IgY Antibodies Reduce Pathological Allergic Responses in Guinea Pigs with Allergic Rhinitis
| Autor: | Wu Xiao-mu, He Dan, Wang Wen-ding, Zhu Xi-ling, Hu Guo-zhu, Xiang Qin, Wen Zhu, Wu Li-hua, Zhou Wen-yun, Wei Hui-ping, Hu Wei-xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Article Subject Guinea Pigs Interleukin-1beta Immunology Immunoglobulins Mucous membrane of nose Fluticasone propionate Allergic inflammation 03 medical and health sciences Edema lcsh:Pathology Animals Medicine Lung medicine.diagnostic_test Tumor Necrosis Factor-alpha business.industry Cell Biology Eosinophil Rhinitis Allergic Disease Models Animal Nasal Mucosa 030104 developmental biology Bronchoalveolar lavage medicine.anatomical_structure Nasal administration Nasal Lavage Fluid medicine.symptom business Research Article lcsh:RB1-214 medicine.drug |
| Zdroj: | Mediators of Inflammation, Vol 2016 (2016) Mediators of Inflammation |
| ISSN: | 0962-9351 |
| DOI: | 10.1155/2016/3128182 |
| Popis: | This study aims to determine whether the combined blockade of IL-1βand TNF-αcan alleviate the pathological allergic inflammatory reaction in the nasal mucosa and lung tissues in allergic rhinitis (AR) guinea pigs. Healthy guinea pigs treated with saline were used as the healthy controls. The AR guinea pigs were randomly divided into (1) the AR model group treated with intranasal saline; (2) the 0.1% nonspecific IgY treatment group; (3) the 0.1% anti-TNF-αIgY treatment group; (4) the 0.1% anti-IL-1βIgY treatment group; (5) the 0.1% combined anti-IL-1βand TNF-αIgY treatment group; and (6) the fluticasone propionate treatment group. The inflammatory cells were evaluated using Wright’s staining. Histopathology was examined using hematoxylin-eosin staining. The results showed that the number of eosinophils was significantly decreased in the peripheral blood, nasal lavage fluid, and bronchoalveolar lavage fluid (P<0.05), and eosinophil, neutrophil, and lymphocyte infiltration and edema were significantly reduced or absent in the nasal mucosa and lung tissues (P<0.05) in the combined 0.1% anti-IL-1β- and TNF-αIgY-treated guinea pigs. The data suggest that topical blockade of IL-1βand TNF-αcould reduce pathological allergic inflammation in the nasal mucosa and lung tissues in AR guinea pigs. |
| Databáze: | OpenAIRE |
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