Genotyping versus phenotyping of non-ABO erythrocyte antigens in patients with the Mediterranean hemopathic syndromes: Effect of transfusion therapy

Autor: Khalid I Elsayh, Safaa A. A. Khaled, Maha Atwa, Marwa M. Thabet, Eman NasrEldin, Nada O. Abdelhameed, Sahar A. Elgammal
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Blood transfusion
Physiology
medicine.medical_treatment
030204 cardiovascular system & hematology
Pathology and Laboratory Medicine
Serology
0302 clinical medicine
Animal Cells
Red Blood Cells
Medicine and Health Sciences
Medicine
Liquid Chromatography
Multidisciplinary
Incidence (epidemiology)
Chromatographic Techniques
Hematology
Clinical Laboratory Sciences
Body Fluids
Phenotype
Blood
Cellular Types
Anatomy
Research Article
Adult
Genotyping
medicine.medical_specialty
Genotype
Science
Research and Analysis Methods
ABO Blood-Group System
03 medical and health sciences
Antigen
Diagnostic Medicine
Internal medicine
ABO blood group system
Humans
Blood Transfusion
Molecular Biology Techniques
Molecular Biology
Blood Cells
Transfusion Medicine
business.industry
Biology and Life Sciences
Transfusion History
Cell Biology
High Performance Liquid Chromatography
Hemoglobinopathies
Transfusion therapy
business
Blood Groups
030215 immunology
Zdroj: PLoS ONE, Vol 16, Iss 7, p e0251576 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: The Mediterranean hemopathic syndromes (MHS) are the most prevalent hemoglobinopathies in the Mediterranean basin. Transfusion therapy is the main therapy for these disorders, particularly for severe forms of the disease. Currently, pre-transfusion serological typing of erythrocyte antigens is the standard tool for reducing complications of transfusion in those patients. This study compared genotyping with phenotyping of non-ABO erythrocyte antigens in patients with MHS and assessed the effect of transfusion therapy on their results. One-hundred ninety-eight MHS patients were recruited, screened, and proven negative for allo-antibodies. They were grouped into two groups: (1) 20 newly diagnosed patients with no transfusion history and (2) 178 previously diagnosed patients undergoing transfusion therapy. Patients were interviewed and clinically examined. Full blood count (FBC) and high performance liquid chromatography (HPLC) were done for group 1 only. Genotyping and phenotyping of non-ABO erythrocyte antigens were performed for group 1, and 25 patients out of group 2 were propensity score-matched (PSM) with group 1. Both groups were gender and age matched; 55% and 74% of groups 1 and 2 had major disease, respectively. Insignificant differences were observed between genotyping and phenotyping of non-ABO erythrocyte antigens in group 1, while significant discrepancies and mixed field results were noted in group 2 patients. Discrepancies were obvious with JKa, JKb, and little c antigens. Conclusively, molecular typing is a powerful tool for pre-transfusion testing in chronically transfused MHS patients. This testing reduces incidence of transfusion reactions. JKa, JKb and little c antigens are the most clinically significant non-ABO erythrocyte antigens.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje