Electrospun aligned nanofibrous composite of MWCNT/polyurethane to enhance vascular endothelium cells proliferation and function
| Autor: | Xiaojin Qi, Haiyan Xu, Sishen Xie, Zhaozhao Han, Hua Kong, Chaoying Wang, Jie Meng |
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| Rok vydání: | 2010 |
| Předmět: |
Collagen Type IV
rho GTP-Binding Proteins Materials science Polyurethanes Biomedical Engineering Nanofibers CDC42 Thromboplastin Biomaterials Type IV collagen Plasminogen Activator Inhibitor 1 Extracellular Humans Composite material Cytoskeleton cdc42 GTP-Binding Protein Cell Shape Cell Proliferation Tissue Engineering Tissue Scaffolds Cell growth Nanotubes Carbon Metals and Alloys Anticoagulants Endothelial Cells Endothelial stem cell Enzyme Activation Cytoplasm Ceramics and Composites Biophysics Laminin Signal transduction |
| Zdroj: | Journal of biomedical materials research. Part A. 95(1) |
| ISSN: | 1552-4965 |
| Popis: | Aligned or random nanofibrous meshes of multiwalled carbon nanotubes/polyurethane composite (MWCNT/PU) were fabricated by electrospinning and characterized by scanning electron microscopy (SEM). The regulatory effects of nanofibrous structure and MWCNT on the growth and anticoagulant function of human umbilical vein endothelial cells (HUVECs) were investigated by examining proliferation, type IV collagen secretion, tissue factor and plasminogen activator inhibitor-1 (PAI-1) release, and cytoskeleton arrangement, as well as via pull-down analysis. We show that aligned nanofibrous structure and MWCNT can function as extracellular signals to stimulate cell growth, proliferation, and extracellular collagen secretion, in addition to preserving anticoagulant function. The nanofibrous structures played important roles in the activation of Rac and Cdc42, while CNT regulated the activation of Rho. These two features synergistically activated Rho GTPases that transmitted cell-substrate signals to the cytoplasm. These signals were then relayed to the nucleus by the MAP kinase pathway to direct cytoskeletal arrangement and cell orientation. |
| Databáze: | OpenAIRE |
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