Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration

Autor:	Camila Fernanda Zorzella Creste, Marjorie de Assis Golim, Patrícia Rodrigues Orsi, Benedito Barraviera, Luis A. Justulin, Fernanda da Cruz Landim-Alvarenga, Rui Seabra Ferreira
Přispěvatelé:	Universidade Estadual Paulista (Unesp)
Rok vydání:	2020
Předmět:	Scaffold 02 engineering and technology Bone healing scaffold lcsh:Technology Article Fibrin 03 medical and health sciences General Materials Science fibrin lcsh:Microscopy Bone regeneration lcsh:QC120-168.85 030304 developmental biology 0303 health sciences lcsh:QH201-278.5 biology lcsh:T Chemistry Regeneration (biology) Mesenchymal stem cell biomaterial bioproduct 021001 nanoscience & nanotechnology Cell biology stem cell lcsh:TA1-2040 Fibrin scaffold biology.protein lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering Stem cell lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology lcsh:TK1-9971
Zdroj:	Materials Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Materials, Vol 13, Iss 2747, p 2747 (2020) Volume 13 Issue 12
ISSN:	1996-1944
DOI:	10.3390/ma13122747
Popis:	Fibrin scaffold fits as a provisional platform promoting cell migration and proliferation, angiogenesis, connective tissue formation and growth factors stimulation. We evaluated a unique heterologous fibrin biopolymer as scaffold to mesenchymal stem cells (MSCs) to treat a critical-size bone defect. Femurs of 27 rats were treated with fibrin biopolymer (FBP) FBP + MSCs and FBP + MSC differentiated in bone lineage (MSC-D). Bone repair was evaluated 03, 21 and 42 days later by radiographic, histological and scanning electron microscopy (SEM) imaging. The FBP + MSC-D association was the most effective treatment, since newly formed Bone was more abundant and early matured in just 21 days. We concluded that FBP is an excellent scaffold for MSCs and also use of differentiated cells should be encouraged in regenerative therapy researches. The FBP ability to maintain viable MSCs at Bone defect site has modified inflammatory environment and accelerating their regeneration.
Databáze:	OpenAIRE
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