Efficient export of secretory proteins through a vacuolized golgi complex
| Autor: | Nina Gahmberg, Leena Peltonen |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Immunoprecipitation
Bunyaviridae Mutant Fluorescent Antibody Technique Golgi Apparatus Chick Embryo symbols.namesake Cricetinae Animals Secretion Immunosorbent Techniques biology Uukuniemi virus Biological Transport Cell Biology Golgi apparatus Fibronectins Cell biology Organoids Fibronectin Hydroxyproline Procollagen peptidase Secretory protein Mutation Vacuoles biology.protein symbols Electrophoresis Polyacrylamide Gel Procollagen |
| Zdroj: | Cell Biology International Reports. 11:547-555 |
| ISSN: | 0309-1651 |
| DOI: | 10.1016/0309-1651(87)90017-8 |
| Popis: | The transport of the secretory proteins fibronectin (FN) and procollagen (PC) was studied in cells infected with the temperaturesensitive mutant ts 12 of Uukuniemi virus. Using pulse-labeling followed by immunoprecipitation and SDS-PAGE (FN), or by determination of radioactivity incorporated into hydroxyproline (PC) at different time points we could show that the secretion rates for these proteins were normal although the Golgi complex had become vacuolized as a result of infection with the virus. We conclude that such a morphologically altered Golgi can still carry out effective transport of secretory proteins. |
| Databáze: | OpenAIRE |
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