Silencing of LncRNA-PVT1 ameliorates lipopolysaccharide-induced inflammation in THP-1-derived macrophages via inhibition of the p38 MAPK signaling pathway
| Autor: | Shengcai Zheng, Canxia Huang, Zhijie He, Yu-Kai Zheng, Zijun Zou, Wenhua Liao, Minggen Zhou, Weichao Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Inflammation
Lipopolysaccharides Advanced and Specialized Nursing MAPK/ERK pathway Gene knockdown MAP Kinase Signaling System business.industry Macrophages p38 mitogen-activated protein kinases p38 Mitogen-Activated Protein Kinases Cell biology Anesthesiology and Pain Medicine Intensive care medicine Humans Gene silencing RNA Long Noncoding Tumor necrosis factor alpha Signal transduction medicine.symptom business Signal Transduction |
| Zdroj: | Annals of Palliative Medicine. 10:6410-6418 |
| ISSN: | 2224-5839 2224-5820 |
| DOI: | 10.21037/apm-21-1078 |
| Popis: | Background Sepsis is common in intensive care units and has a high mortality rate; yet, its pathogenesis and treatment remain unclear. Recent studies have shown that long non-coding RNA plasmacytoma variant translocation 1 (lncRNA-PVT1) plays a pro-inflammatory role in immune-related inflammatory diseases. Therefore, we investigated whether lncRNA-PVT1 plays an important pro-inflammatory effect in the inflammatory response of sepsis. Methods Quantitative real-time PCR (RT-qPCR) was employed for the detection of lncRNA-PVT1, interleukin 1β (IL-1β), and tumor necrosis factor α (TNF-α) mRNA, and the correlations between their expressions were analyzed. After lncRNA-PVT1 knockdown by lncRNA Smart Silencer, abnormal expressions of lncRNA-PVT1, and IL-1β and TNF-α mRNA were detected. The expressions of total and phosphorylated protein of p38 were detected by western blotting. The effect of silencing lncRNA-PVT1 on p38 mitogen-activated protein kinase (MAPK) signaling pathway during lipopolysaccharide (LPS)-induced inflammation was subsequently analyzed. The MAPK selective inhibitor, SB202190, was used to block this signaling pathway, and the expressions of lncRNA-PVT1 and TNF-α were detected by RT-qPCR. Furthermore, the effect of partial blockade of the p38 MAPK signaling pathway by SB202190 on the levels of lncRNA-PVT1 was explored. Results Following treatment of THP-1-derived macrophages with different concentrations of LPS, the levels of lncRNA-PVT1 and IL-1β, TNF-α mRNA were increased in a dose-dependent manner. Silencing of lncRNA-PVT1 reduced the expressions of IL-1β and TNF-α mRNA via inhibition of the p38 MAPK signaling pathway. Specifically, inhibiting the p38 MAPK pathway significantly decreased the LPS-induced lncRNA-PVT1 elevation. Conclusions Our observations suggest that lncRNA-PVT1 can be silenced to ameliorate LPS-induced inflammation in macrophages via inhibition of the p38 MAPK pathway. Further, the p38 MAPK pathway can regulate the expression of lncRNA-PVT1 via a positive feedback loop. |
| Databáze: | OpenAIRE |
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