Gating strategy for APC characterization from Combination Therapy with Anti-PD-1, Anti-TIM-3, and Focal Radiation Results in Regression of Murine Gliomas

Autor: Michael Lim, Drew M. Pardoll, Henry Brem, Charles G. Drake, Peter C. Burger, Janis M. Taube, Alessandro Olivi, Xiaobu Ye, Phuoc T. Tran, Betty M. Tyler, Allison M. Martin, Mary Sheu, Tomas Garzon-Muvdi, Sarah Harris-Bookman, Christopher M. Jackson, Dimitrios Mathios, Haiying Xu, Ada Tam, Eric W. Sankey, Ann Liu, Esteban Velarde, Debebe Theodros, Eileen S. Kim, Antonella Mangraviti, Mira A. Patel, Jennifer E. Kim
Rok vydání: 2023
Popis: Cells were first gated on size and singularity by forward scatter and side scatter. Nonviable cells were excluded by live/dead gating. Live cells were gated on CD11c and CD11b, then on CD45 and F4/80 to identify macrophage and dendritic cell phenotypes. Finally myeloid cells were gated for expression of TIM-3. Final populations were (A) F4/80+CD45hiCD11c-CD11bhi (B) F4/80+CD45dimCD11bhi (C) F4/80+CD45hiCD11c+CD11b+ and (D) F4/80-CD45dimCD11c+CD11b-.
Databáze: OpenAIRE