Silencing of circ_0000517 suppresses proliferation, glycolysis, and glutamine decomposition of non‐small cell lung cancer by modulating miR ‐330‐5p/ YY1 signal pathway
| Autor: | Tian-Ge Wang, Yanqing Wang, Jiaqi Zhang, Guige Wang, Zhong-Xing Bing, Dan-Qing Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Medicine (General) Lung Neoplasms Glutamine NSCLC YY1 Mice R5-920 Circular RNA Carcinoma Non-Small-Cell Lung Cell Line Tumor medicine Animals Humans Gene silencing Glycolysis miR‐330‐5p Lung cancer YY1 Transcription Factor Aged Cell Proliferation circ_0000517 Gene knockdown biology business.industry Cancer RNA Circular General Medicine Middle Aged glycolysis medicine.disease respiratory tract diseases Proliferating cell nuclear antigen MicroRNAs Cancer research biology.protein Female business Signal Transduction |
| Zdroj: | Kaohsiung Journal of Medical Sciences, Vol 37, Iss 12, Pp 1027-1037 (2021) |
| ISSN: | 2410-8650 1607-551X |
| DOI: | 10.1002/kjm2.12440 |
| Popis: | In recent years, circular RNA (circRNA) has been found to be involved in a variety of cancer processes. More and more attention has been paid to the research of circRNA in lung cancer. This study aims to investigate whether circ_0000517 affected the physiology of non‐small cell lung cancer (NSCLC) and the underlying mechanism. The results demonstrated that circ_0000517 was highly expressed in lung cancer tissues and cells, and overexpression of circ_0000517 was negatively correlated with the prognosis of NSCLC patients. Silencing of circ_0000517 significantly inhibited the proliferation, glycolysis, and glutamine decomposition of NSCLC cells in vitro and repressed the growth of xenografted tumors in vivo. Moreover, knockdown of circ_0000517 attenuated the expression of PCNA, HK2, LDHA, ASCT2, and GLS1. Further study found that circ_0000517 targeted miR‐330‐5p and miR‐330‐5p targeted YY1. In addition, miR‐330‐5p inhibitor reversed inhibition of cancer cell proliferation, glycolysis, and glutamine decomposition induced by si‐circ_0000517. In conclusion, our study revealed that silencing of circ_0000517 improved the progression of NSCLC through regulating miR‐330‐5p/YY1 axis. |
| Databáze: | OpenAIRE |
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