Micro and nanoscale characterization of poly(DL-lactic-co-glycolic acid) films subjected to the L929 cells and the cyclic mechanical load
| Autor: | Adrian Chlanda, Wojciech Swieszkowski, Alicja Kosik-Kozioł, Emilia Choińska, Jakub Jaroszewicz, Marcin Heljak, Maryla Moczulska-Heljak, Tomasz Jaroszewicz |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Kinetics General Physics and Astronomy Young's modulus Biodegradable Plastics macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences Cell Line Mice symbols.namesake chemistry.chemical_compound L Cells Polylactic Acid-Polyglycolic Acid Copolymer Structural Biology Absorbable Implants Surface roughness Animals General Materials Science Glycolic acid Cell Proliferation Mechanical load technology industry and agriculture Cell Biology Fibroblasts 021001 nanoscience & nanotechnology 0104 chemical sciences Amorphous solid PLGA chemistry Chemical engineering symbols 0210 nano-technology Glass transition |
| Zdroj: | Micron. 115:64-72 |
| ISSN: | 0968-4328 |
| DOI: | 10.1016/j.micron.2018.09.004 |
| Popis: | In this paper, the effect of the presence of L929 fibroblast cells and a cyclic load application on the kinetics of the degradation of amorphous PLGA films was examined. Complex micro and nano morphological, mechanical and physico-chemical studies were performed to assess the degradation of the tested material. For this purpose, molecular weight, glass transition temperature, specimen morphology (SEM, μCT) and topography (AFM) as well as the stiffness of the material were measured. The study showed that the presence of living cells along with a mechanical load accelerates the PLGA degradation in comparison to the degradation occurring in acellular media: PBS and DMEM. The drop in molecular weight observed was accompanied by a distinct increase in the tensile modulus and surface roughness, especially in the case of the film degradation in the presence of cells. The suspected cause of the rise in stiffness during the degradation of PLGA films is a reduction in the molecular mobility of the distinctive superficial layer resulting from severe structural changes caused by the surface degradation. In conclusion, all the micro and nanoscale properties of amorphous PLGA considered in the study are sensitive to the presence of L929 cells, as well as to a cyclic load applied during the degradation process. |
| Databáze: | OpenAIRE |
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