Morphological and functional studies of rat hepatocytes on a hydrophobic or hydrophilic polydimethylsiloxane surface
| Autor: | Kohji Nakazawa, Ryuhei Mori, Yumiko Izumi |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Materials science Morphology (linguistics) Surface Properties Biomedical Engineering Biochemistry Culture Media Serum-Free Biomaterials chemistry.chemical_compound Monolayer Cell Adhesion Animals Dimethylpolysiloxanes Rats Wistar Cell adhesion Molecular Biology Cells Cultured Polydimethylsiloxane Cadherin Cell adhesion molecule Spheroid General Medicine Rats Blood chemistry Hepatocytes Biophysics Wetting Biotechnology |
| Zdroj: | Acta Biomaterialia. 5:613-620 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2008.08.011 |
| Popis: | This study describes the morphological and functional behavior of rat hepatocytes on a polydimethylsiloxane (PDMS)-coated surface. Hepatocytes were cultured on hydrophobic or hydrophilic PDMS-coated surfaces in serum-free and serum-containing media. In the serum-free medium, almost all hepatocytes adhered onto the surface irrespective of the wettability, with a cell adhesion ratio of >90% at 24h. In the serum-containing medium, although they strongly adhered onto the hydrophilic surface (cell adhesion ratio >85%), the ratio on the hydrophobic surface was |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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