A role ofmiR-27in the regulation of adipogenesis
| Autor: | Zhanguo Gao, Qun Lin, Zhong Yun, Rodolfo M. Alarcon, Jianping Ye |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Mice Obese Adipose tissue Biology Transfection Biochemistry Article Energy homeostasis Mice chemistry.chemical_compound 3T3-L1 Cells Internal medicine Adipocyte microRNA medicine Animals Epigenetics Molecular Biology Cells Cultured Adipogenesis miR-27 Cell Differentiation Cell Biology Cell biology PPAR gamma MicroRNAs Endocrinology chemistry Homeostasis |
| Zdroj: | FEBS Journal. 276:2348-2358 |
| ISSN: | 1742-4658 1742-464X |
| DOI: | 10.1111/j.1742-4658.2009.06967.x |
| Popis: | MicroRNAs (miRNAs) are involved in a plethora of important biological processes, from embryonic development to homeostasis in adult tissues. Recently, miRNAs have emerged as a class of epigenetic regulators of metabolism and energy homeostasis. We have investigated the role of miRNAs in the regulation of adipogenic differentiation. In this article, we demonstrate that the miR-27 gene family is downregulated during adipogenic differentiation. Overexpression of miR-27 specifically inhibited adipocyte formation, without affecting myogenic differentiation. We also found that expression of miR-27 resulted in blockade of expression of PPARgamma and C/EBPalpha, the two master regulators of adipogenesis. Importantly, expression of miR-27 was increased in fat tissue of obese mice and was regulated by hypoxia, an important extracellular stress associated with obesity. Our data strongly suggest that miR-27 represents a new class of adipogenic inhibitors and may play a role in the pathological development of obesity. |
| Databáze: | OpenAIRE |
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