Interleukin-6 Inhibits Regulatory T Cells and Improves the Proliferation and Cytotoxic Activity of Cytokine-induced Killer Cells
| Autor: | Lian Li, Shengping Li, Jun Wang, Alfred E. Chang, Xiang-Ming Lao, Guohe Lin, Qiao Li, S. W. Li, Jin Wang, Yong Dong, Jinye Zhang |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Cytotoxicity Immunologic Male Cancer Research Carcinoma Hepatocellular Immunology chemical and pharmacologic phenomena T-Lymphocytes Regulatory Cell Line Immunophenotyping Interleukin 21 Cytokine-Induced Killer Cells Humans Immunology and Allergy IL-2 receptor Antigen-presenting cell Cells Cultured Aged Cell Proliferation Interleukin 3 Pharmacology Lymphokine-activated killer cell Cytokine-induced killer cell Interleukin-6 Chemistry Liver Neoplasms Middle Aged Natural killer T cell Interleukin 12 Cancer research Female |
| Zdroj: | Journal of Immunotherapy. 35:337-343 |
| ISSN: | 1524-9557 |
| DOI: | 10.1097/cji.0b013e318255ada3 |
| Popis: | The presence of regulatory T cells in patients who received therapeutic cytokine-induced killer (CIK) cells may inhibit host immunity, leading to failed immunotherapy. In this study, we investigated the impact of using interleukin-6 (IL-6) on the phenotype alteration, proliferation, and cytotoxic activity of CIK cells generated from the peripheral blood mononuclear cells of patients with hepatocellular carcinoma. We found that addition of IL-6 to CIK-cell culture medium decreased the percentage of Treg/CD4(+), Treg/CD3(+) T cells in the resultant CIK cells and simultaneously increased the proliferation ability, the expression of CD45RO(+)CD62L(low)CCR7(low) effector memory phenotype, and cytotoxicity of the CIK cells against hepatocellular carcinoma in vitro. Our results also showed that the percentage of Th17/CD4(+) cells was increased in CIK cells, but the proportion of Th17/CD4(+) cells was not affected by the addition of IL-6 to CIK-cell culture medium. Collectively, these data suggest that IL-6 may have the potential to improve antitumor activity of CIK cells in cancer immunotherapy. |
| Databáze: | OpenAIRE |
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