Analytical performance of a triple quadrupole mass spectrometer compared to a high resolution mass spectrometer for the analysis of polybrominated diphenyl ethers in fish
| Autor: | Alicia Pérez-Fuentetaja, Diana S. Aga, Susan A. Mackintosh, Lisa R. Zimmerman, Mark D. Clapsadl, Grazina Pacepavicius, Mehran Alaee |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Resolution (mass spectrometry)
Mass spectrometry Tandem mass spectrometry Sensitivity and Specificity Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry Matrix (chemical analysis) Polybrominated diphenyl ethers Limit of Detection Tandem Mass Spectrometry Halogenated Diphenyl Ethers Animals Humans Environmental Chemistry Spectroscopy Flame Retardants Chromatography biology Chemistry Selected reaction monitoring Fishes biology.organism_classification Triple quadrupole mass spectrometer Trout Environmental chemistry |
| Zdroj: | Analytica Chimica Acta. 747:67-75 |
| ISSN: | 0003-2670 |
| Popis: | Polybrominated diphenyl ethers (PBDEs) are a class of flame retardants used globally in many consumer products and industrial applications. Traditionally, gas chromatography-high resolution mass spectrometry (GC-HR-MS) is the method of choice for analysis of PBDEs in environmental samples because it offers high sensitivity and selectivity, resulting in less interferences. However, the specificity offered by gas chromatography-triple quadrupole tandem mass spectrometry (GC-QQQ-MS/MS), operated in selected reaction monitoring mode, provides a more affordable alternative to GC-HR-MS for the analysis of PBDEs in complex environmental samples. In this study, an analytical method was developed for the analysis of 41 PBDE congeners in fish using GC-QQQ-MS/MS. Results from the analysis of three fish species [lake trout (Salvelinus namaycush), yellow perch (Perca flavescens), and round goby (Neogobius melanostomus)] using GC-QQQ-MS/MS were compared with those obtained by GC-HR-MS. These species were selected because they represent varying levels of lipid-rich matrix and contaminant loads. Instrumental limits of detection for the GC-QQQ-MS/MS ranged from 0.04 pg to 41 pg, whereas those for the GC-HR-MS ranged from 5 pg to 85 pg. The PBDE values obtained from these two methods were highly correlated, R(2) values >0.7, for all three fish species, supporting the suitability of GC-QQQ-MS/MS for analysis of PBDEs in fish with varying fat content. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect