Attenuation of periostin in retinal Müller glia by TNF-α and IFN-γ
| Autor: | Song Bai Jia, Hui Lan Zeng, Shigeo Yoshida, Lu Si Zhang, Ying Qian Peng, Jing Ling Zou, Jing Ming Shi, Takahito Nakama, Yedi Zhou, Man Jing Cao, Yoshiyuki Kobayashi |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
müller glia
Periostin ifn-γ Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Ophthalmology medicine periostin Gene knockdown Retina tnf-α business.industry Retinal eye diseases Cell biology Reverse transcription polymerase chain reaction retinal neovascularization Ophthalmology Vascular endothelial growth factor A medicine.anatomical_structure Basic Research chemistry lcsh:RE1-994 030221 ophthalmology & optometry sense organs business Muller glia |
| Zdroj: | International Journal of Ophthalmology, Vol 12, Iss 2, Pp 212-218 (2019) |
| ISSN: | 2227-4898 2222-3959 |
| Popis: | Aim To investigate the regulation and mechanisms of periostin expression in retinal Muller glia, and to explore the relevance to retinal neovascularization. Methods The oxygen-induced retinopathy (OIR) mouse model and the human Moorfield/Institute of Ophthalmology-Muller 1 (MIO-M1) cell line were used in the study. Immunofluorescence staining was used to determine the distribution and expression of periostin and a Muller glial cell marker glutamine synthetase (GS). Cytokines TNF-α and IFN-γ were added to stimulate the MIO-M1 cells. ShRNA was used to knockdown periostin expression in MIO-M1 cells. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was conducted to assess the mRNA expression of periostin. Results Immunofluorescence staining showed that periostin was expressed by MIO-M1 Muller glia. GS-positive Muller glia and periostin increased in OIR retinas, and were partially overlaid. The stimulation of TNF-α and IFN-γ reduced the mRNA expression of periostin significantly and dose-dependently in MIO-M1 cells. Knockdown of periostin reduced mRNA expression of vascular endothelial growth factor A (VEGFA) in MIO-M1 cells, while VEGFA expression was not changed in periostin knock-out OIR retinas. Conclusion Muller glia could be one of the main sources of periostin in the retina, and might contribute to the pathogenesis of retinal neovascularization. Proinflammatory cytokines TNF-α and IFN-γ attenuate the periostin expression in retinal Muller glia, which provides a potential and novel method in treating retinal neovascular diseases. |
| Databáze: | OpenAIRE |
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