Dietary Pseudopurpurin Effects on Bone Mineral Density and Bone Geometry Architecture in Rats
| Autor: | Tie-Suo Han, Guowen Liu, Xiaobing Li, Wei-Zhong Wang, Zhe Wang, Chen-Chen Wu, Peng Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
X-ray microtomography Bone density Osteoporosis chemistry.chemical_element Anthraquinones Calcium Bone and Bones Article Catalysis Inorganic Chemistry lcsh:Chemistry Calcification Physiologic Bone Density Internal medicine medicine Animals Humans pseudopurpurin Femur Rats Wistar Physical and Theoretical Chemistry Coloring Agents Molecular Biology lcsh:QH301-705.5 Spectroscopy Bone mineral Bone geometry bone mineral elements Body Weight Organic Chemistry X-Ray Microtomography General Medicine Anatomy medicine.disease Rats Computer Science Applications Calcium Dietary Endocrinology chemistry lcsh:Biology (General) lcsh:QD1-999 Dietary Supplements Female Histopathology bone mineral density bone geometry architecture |
| Zdroj: | International Journal of Molecular Sciences, Vol 13, Iss 3, Pp 3431-3443 (2012) International Journal of Molecular Sciences Volume 13 Issue 3 Pages 3431-3443 |
| ISSN: | 1422-0067 |
| Popis: | The objective of our study was to evaluate whether feeding pseudopurpurin affects bone mineral density and bone geometry architecture in rats. Pseudopurpurin was extracted, analyzed and purified using an HLPC-ESI-MS. Rats were given 0% and 0.5% pseudopurpurin powder in their diet. Femurs of rats were examined at 0.5, 1 and 2 months after pseudopurpurin feeding. Compared with rats in the group 0%, the bone mineral density, and the calcium, magnesium, zinc and manganese concentrations in the rats femur in the group 0.5% increased significantly at 1 month and 2 months after pseudopurpurin feeding. Analytical results of micro-computed tomography showed that the group 0.5% displayed an increase in the trabecular volume fraction, trabecular thickness and trabecular number of the distal femur at 1 and 2 months after pseudopurpurin feeding, and the mean thickness, inner perimeter, outer perimeter, and area of the femur diaphysis were significantly increased at 2 months after pseudopurpurin feeding compared with the group 0%. In parallel, the trabecular separation and structure model index of the distal femur were decreased, compared with the group 0% at 1 and 2 months after pseudopurpurin feeding. In the 0.5% and 0% groups, there was no damage to kidney and liver by histopathology analysis. The long-term feeding of pseudopurpurin is safe for rats. The feeding of 0.5% pseudopurpurin which has specific chemical affinities for calcium for bone improvement and level of bone mineral density, enhances the geometry architecture compared with the 0% group. |
| Databáze: | OpenAIRE |
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