Immunodensity and mRNA expression of A2A adenosine, D2 dopamine, and CB1 cannabinoid receptors in postmortem frontal cortex of subjects with schizophrenia: effect of antipsychotic treatment

Autor: Rafael Franco, Romano La Harpe, Leyre Urigüen, M. Julia García-Fuster, Carmen Lluis, Luis F. Callado, Benito Morentin, Vicent Casadó, J. Javier Meana, Jesús A. García-Sevilla
Rok vydání: 2009
Předmět:
Adult
Male
Psychosis
medicine.medical_specialty
Receptor
Adenosine A2A
medicine.medical_treatment
RNA
Messenger/metabolism
Schizophrenia/drug therapy/metabolism/pathology
Antipsychotic Agents/pharmacology/therapeutic use
Pharmacology
chemistry.chemical_compound
Young Adult
Receptor
Cannabinoid
CB1
Dopamine
Dopamine receptor D2
Internal medicine
mental disorders
medicine
Humans
RNA
Messenger
Receptor
Neurotransmitter
Suicide/psychology
Aged
Analysis of Variance
Receptor
Adenosine A2A/genetics/metabolism
Receptors
Dopamine D2
ddc:614.1
Middle Aged
medicine.disease
Receptors
Dopamine D2/genetics/metabolism
Frontal Lobe
Gene Expression Regulation/drug effects/genetics
Suicide
Endocrinology
chemistry
Frontal Lobe/drug effects
Gene Expression Regulation
Schizophrenia
Dopamine receptor
Postmortem Changes
Receptor
Cannabinoid
CB1/genetics/metabolism
Female
Cannabinoid
Psychology
medicine.drug
Antipsychotic Agents
Zdroj: Psychopharmacology, Vol. 206, No 2 (2009) pp. 313-24
ISSN: 1432-2072
Popis: RATIONALE: Dopamine D2 receptors are the main target of antipsychotic drugs. In the brain, D2 receptors coexpress with adenosine A2A and CB1 cannabinoid receptors, leading to functional interactions. OBJECTIVES: The protein and messenger RNA (mRNA) contents of A2A, D2, and CB1 receptors were quantified in postmortem prefrontal cortex of subjects with schizophrenia. MATERIALS AND METHODS: The study was performed in subjects suffering schizophrenia (n=31) who mainly died by suicide, matched with non-schizophrenia suicide victims (n=13) and non-suicide controls (n=33). The density of receptor proteins was evaluated by immunodetection techniques, and their relative mRNA expression was quantified by quantitative real-time polymerase chain reaction. RESULTS: In schizophrenia, the densities of A2A (90+/-6%, n=24) and D2-like receptors (95+/-5%, n=22) did not differ from those in controls (100%). Antipsychotic treatment did not induce changes in the protein expression. In contrast, the immunodensity of CB1 receptors was significantly decreased (71+/-7%, n=11; p
Databáze: OpenAIRE
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