Interleukin-8 Stimulates Calcium Transients and Promotes Epidermal Cell Proliferation
| Autor: | Charles Lam, Alexander G. Haslberger, Ivan. Lindley, Andrea. Tuschil |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Keratinocytes
medicine.medical_specialty TGF alpha Neutrophils chemistry.chemical_element Dermatology Calcium Biology Biochemistry chemistry.chemical_compound Epidermal growth factor Internal medicine medicine Humans Interleukin 8 Molecular Biology Cells Cultured Epidermis (botany) Interleukin-8 DNA Cell Biology Cell biology EGTA HaCaT Endocrinology chemistry Epidermal Cells Epidermis Intracellular Cell Division |
| Zdroj: | Journal of Investigative Dermatology. 99(3):294-298 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12616634 |
| Popis: | The presence of large amounts of biologically active interleukin-8 (IL-8) in psoriatic involved skin suggests that it may contribute, in part, to the changes observed in psoriasis, including hyperproliferation of keratinocytes. To examine the effect of IL-8 on epidermal growth, we monitored cytosolic free Ca ++ transients in human keratinocytes adult skin epidermis calcium reduced level, temperature elevated (HaCat) cells and normal keratinocytes loaded with the cell permeable, acetoxymethyl derivative, indo-1AM. Addition of IL-8 (0.06–47 nM) to the HaCat cells induced rapid rises in cytosolic free Ca ++ from resting levels of 145±38 to peak levels of 889±10 nM. The induced rises in Ca ++ were transient and concentration dependent. Half maximal effect was observed at 1.2 nM. Normal keratinocytes also responded to IL-8 (6 nM) by rises in cytosolic free Ca ++ from a pre-stimulated level of 269 nM to transient peak value of 393 nM. In addition, IL-8 promoted epidermal cell proliferation. Polyclonal anti-IL-8 antibody blocked IL-8-induced calcium changes and proliferation. Under similar conditions, human neutrophils also responded to IL-8 in a similar dose range by a rapid and transient mobilization of Ca ++ . The findings indicate that IL-8 has a wider range of responsive target cells than hitherto thought and acts as an autocrine growth factor. |
| Databáze: | OpenAIRE |
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