Detection of respiratory viruses in gargle specimens of healthy children
| Autor: | Tetsuo Kase, Satoshi Hiroi, Saeko Morikawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
RVB rhinovirus genogroup B Rhinovirus viruses Parechovirus medicine.disease_cause Polymerase Chain Reaction PCR polymerase chain reaction Healthy children Child Asymptomatic Infections Respiratory Tract Infections Coronavirus hMPV human metapneumovirus Enterovirus RT reverse transcription Coinfection Human parechovirus virus diseases Parainfluenza Virus 2 Human Respiratory Syncytial Viruses Infectious Diseases medicine.anatomical_structure RVA rhinovirus genogroup A Child Preschool Viruses Female medicine.symptom hCoV human coronavirus hPeV human parechovirus EV enterovirus Biology Real-Time Polymerase Chain Reaction Asymptomatic Virus Article FluV influenza virus Adenoviridae RVC rhinovirus genogroup C Virology hBoV human bocavirus medicine Humans Clinical significance Gargle specimen AdV adenovirus Respiratory viruses PIV parainfluenza virus RV rhinovirus Immunology Pharynx RSV respiratory syncytial virus Respiratory tract Real-time PCR |
| Zdroj: | Journal of Clinical Virology |
| ISSN: | 1873-5967 1386-6532 |
| Popis: | Highlights • Gargle samples were collected from children and tested respiratory viruses. • In 45(45/200; 22.5%) episodes, some respiratory viruses detected without symptoms. • Under asymptomatic conditions, detected viruses were mainly RVs and EV/RV untyped. • PIVs, RSV and hCoV OC43 were detected only when clinical symptom was seen. • Asymptomatic infections may play an important role in the viral circulation. Background Respiratory tract viral infection is one of the most common and important diseases in children. Polymerase chain reaction (PCR) tests are often used to detect viruses in samples, it is difficult to interpret the clinical significance of PCR positivity, which may reflect a past, imminent or active asymptomatic infection due to their high sensitivity. Although single respiratory viruses have been detected in samples from children with symptoms, other respiratory viruses can also be detected simultaneously. However, the clinical importance of these findings for the symptoms is not known. Objectives To investigate the prevalence of respiratory viruses among children without any symptoms such as acute respiratory illness and/or fever. Study design From week twenty-five 2013 to week twenty-six 2014, gargle samples were collected from children once a week and these samples were subjected to real-time PCR to detect respiratory viruses. On each sampling day, we asked the parents about their children’s health condition. Results Among the 286 samples collected, 200 were from asymptomatic children. In the asymptomatic condition, human parechovirus, adenovirus, enterovirus, rhinovirus, coronavirus 229E and HKU1 were observed in 45 episodes. In samples from symptomatic children, parainfluenza viruses, respiratory syncytial virus and coronavirus OC43 were detected in addition to those mentioned above. Conclusions Various viruses of different species were detected in the specimens from the children regardless of their health status. It might be speculated that host factors such as the function of the immune system influence the clinical outcome of the infection. However, this needs to be studied further. |
| Databáze: | OpenAIRE |
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