Production of L-DOPA and dopamine in recombinant bacteria bearing theVitreoscillahemoglobin gene
| Autor: | Emel Aytan, Asli Giray Kurt, Ufuk Ozer, Hikmet Geckil, Burhan Ates |
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| Rok vydání: | 2009 |
| Předmět: |
Hemeproteins
Dopamine Heterologous Tyrosine phenol-lyase Biology Applied Microbiology and Biotechnology law.invention Microbiology Levodopa Bacterial Proteins law Tyrosine Tyrosine Phenol-Lyase Gene chemistry.chemical_classification Truncated Hemoglobins food and beverages General Medicine biology.organism_classification Recombinant Proteins Citrobacter freundii Enzyme chemistry Recombinant DNA Erwinia Molecular Medicine Bacteria |
| Zdroj: | Biotechnology Journal. 4:1077-1088 |
| ISSN: | 1860-7314 1860-6768 |
| DOI: | 10.1002/biot.200900130 |
| Popis: | Given the well-established beneficial effects of Vitreoscilla hemoglobin (VHb) on heterologous organisms, the potential of this protein for the production of L-DOPA and dopamine in two bacteria, Citrobacter freundii and Erwinia herbicola, was investigated. The constructed recombinants bearing the VHb gene (vgb(+)) had substantially higher levels of cytoplasmic L-DOPA (112 mg/L for C. freundii and 97 mg/L for E. herbicola) than their respective hosts (30.4 and 33.8 mg/L) and the vgb(-) control strains (35.6 and 35.8 mg/L). Further, the vgb(+) recombinants of C. freundii and E. herbicola had 20-fold and about two orders of magnitude higher dopamine levels than their hosts, repectively. The activity of tyrosine phenol-lyase, the enzyme converting L-tyrosine to L-DOPA, was well-correlated to cytoplasmic L-DOPA levels. As cultures aged, higher tyrosine phenol-lyase activity of the vgb(+) strains was more apparent. |
| Databáze: | OpenAIRE |
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