Alpha2-adrenergic receptor-mediated release of lysophosphatidic acid by adipocytes. A paracrine signal for preadipocyte growth

Autor: Philippe Valet, Michel Record, Max Lafontan, P. Barbe, Jean Sébastien Saulnier-Blache, Danièle Daviaud, Céline Pagès, Olivier Jeanneton
Přispěvatelé: Unité de recherche sur les obésités, IFR 31 Louis Bugnard (IFR 31), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie de l'adipocyte, physiologie du tissu adipeux et obésités, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut Louis Bugnard-Institut National de la Santé et de la Recherche Médicale (INSERM), Phospholipides membranaires, signalisation cellulaire et lipoprotéines, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM), Saulnier-Blache, Jean Sébastien
Jazyk: angličtina
Rok vydání: 1998
Předmět:
MESH: 3T3 Cells
Adipose tissue
Stimulation
White adipose tissue
chemistry.chemical_compound
Mice
Idazoxan
Adipocyte
Lysophosphatidic acid
Adipocytes
MESH: Receptors
MESH: Animals
Cells
Cultured
Cell Differentiation
MESH: Comparative Study
General Medicine
3T3 Cells
MESH: Idazoxan
Actin Cytoskeleton
Brimonidine Tartrate
MESH: Cell Division
Female
lipids (amino acids
peptides
and proteins)
Cell Division
Research Article
MESH: Cells
Cultured
Adult
MESH: Cell Differentiation
medicine.medical_specialty
Adrenergic receptor
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
MESH: Actins
MESH: Lysophospholipids
Paracrine signalling
MESH: Quinoxalines
Receptors
Adrenergic
alpha-2
Internal medicine
Culture Techniques
Quinoxalines
Paracrine Communication
medicine
Animals
Humans
MESH: Paracrine Communication
MESH: Microfilaments
Autocrine signalling
MESH: Mice
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
MESH: Adipocytes
MESH: Humans
MESH: Adult
Actins
Endocrinology
chemistry
MESH: Culture Techniques
Lysophospholipids
MESH: Female
Zdroj: Journal of Clinical Investigation
Journal of Clinical Investigation, 1998, 101 (7), pp.1431-8
ISSN: 0021-9738
Popis: In the search for the existence of adrenergic regulation of the autocrine/paracrine function of the white adipose tissue, it was observed that conditioned media from isolated adipocytes or dialysates obtained by in situ microdialysis of human subcutaneous adipose tissue increased spreading and proliferation of 3T3F442A preadipocytes. These effects were amplified when an alpha2-adrenergic agonist was present during the obtention of conditioned media and microdialysates. This alpha2-adrenergic-dependent trophic activity was completely abolished by pretreatment of the conditioned media or microdialysates with the lysophospholipase, phospholipase B. Among the different lysophospholipids tested only lysophosphatidic acid (LPA) was able to induce spreading and proliferation of 3T3F442A preadipocytes. Moreover, previous chronic treatment of 3T3F442A preadipocytes with LPA which led to a specific desensitization of LPA responsiveness, abolished the alpha2-adrenergic-dependent trophic activities of the conditioned media and microdialysates. Finally, alpha2-adrenergic stimulation led to a rapid, sustained, and pertussis toxin-dependent release of [32P]LPA from [32P]-labeled adipocytes. Based upon these results it was proposed that in vitro and in situ stimulation of adipocyte alpha2-adrenergic receptors provokes the extracellular release of LPA leading, in turn, to regulation of preadipocyte growth.
Databáze: OpenAIRE
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