Green synthesis of Gold Nanoparticles using Dovyalis abyssinica leave extract and its characterization and functional evaluation
Autor: | Abera Beyene |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: | |
DOI: | 10.20372/nadre/19933 |
Popis: | n this work, gold nanoparticles (Au NPS) are synthesized using aqueous extract of Dovyalis abyssinica (D. abyssinica) leaf as reducing and surface capping agent. The phytochemical test of the crude extract showed the presence of tannins, phenolics, steroids, terpenes and saponins. HAuCl4 was successfully synthesized in the laboratory from pure gold and hydrochloric acid and then Au NPs were synthesized from HAuCl4 and hydrochloric acid at 45 oC with a digestion time of 1 hr. The size of the particle was modulated by varying the ratio of the plant extract and HAuCl4 to obtain the smallest average particle size, 63.13 nm. To study the nature of reducing compounds, the crude water extract was fractionated with hexane and ethyl acetate sequentially. Based on the evaluation results, the aqueous fraction was the best reducing fraction to synthesize Au NPs. The SEM image of the synthesized Au NPs showed mixed shapes which are spherical, cylindrical rods and some irregularities. The synthesis of Au NPs was also confirmed by its characteristic crystalline peaks from X-ray diffraction (XRD) spectra. The FTIR spectrum revealed the presence of primary amines, cyanids, ether linkages sulfide bonds, and aromatic rings indicating the presence of proteins with more functional groups. The synthesized Au NPs showed excellent free radical scavenging potential against 2, 2-Diphenyl-2-picrylhydrazyl (DPPH) and catalytic activity for degradation of Bromothymol blue and methyl red. However, the newly synthesized Au NPs were not effective to inhibit the growth of selected bacteria (Staphylococcus aureus and E.coli). |
Databáze: | OpenAIRE |
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