Molecular cloning and disruption of a novel gene encoding UDP-glucose:tetrahydrobiopterin α-glucosyltransferase in the cyanobacteriumSynechococcussp. PCC 7942

Autor: Yong Kee Choi, Yoon Kyung Hwang, Young Shik Park
Rok vydání: 2001
Předmět:
Glycosylation
Tetrahydrobiopterin-glucoside
Polymerase Chain Reaction
Biochemistry
Structural Biology
Antineoplastic Combined Chemotherapy Protocols
Cloning
Molecular
Chromatography
High Pressure Liquid
Synechocystis sp. PCC 6803
biology
Gene disruption overexpression
Synechocystis
Cytarabine
Tetrahydrobiopterin
Recombinant Proteins
Blotting
Southern
Glucosyltransferases
Electrophoresis
Polyacrylamide Gel
Glucosyltransferase
Pteridine glycosyltransferase
Cyanobacterium
Cell Division
medicine.drug
DNA
Bacterial
Blotting
Western
Molecular Sequence Data
Biophysics
Molecular cloning
Cyanobacteria
Bacterial Proteins
Glycosyltransferase
Escherichia coli
Genetics
medicine
Amino Acid Sequence
Aclarubicin
Molecular Biology
Gene
DNA Primers
Base Sequence
Wild type
Cell Biology
biology.organism_classification
Biopterin
genomic DNA
Genes
Bacterial
Mutation
biology.protein
Zdroj: FEBS Letters. 502:73-78
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(01)02667-9
Popis: The gene encoding UDP-glucose:tetrahydrobiopterin α-glucosyltransferase (BGluT) was cloned from the genomic DNA of Synechococcus sp. PCC 7942. The encoded protein consisting of 359 amino acid residues was verified in vitro and in vivo to be responsible for the synthesis of tetrahydrobiopterin (BH4)-glucoside produced in the organism. The BGluT gene is the first cloned in pteridine glycosyltransferases and also a novel one cloned so far in UDP-glycosyltransferases. The mutant cells disrupted in the BGluT gene produced only aglycosidic BH4 at a level of 8.3% of the BH4-glucoside in wild type cells and exhibited half of the wild type growth in normal photoautotrophic conditions. These results suggest that the glucosylation of BH4 is required for the maintenance of the high cellular concentration of the compound, thereby supporting the normal growth of Synechococcus sp. PCC 7942.
Databáze: OpenAIRE
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