Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein
Autor: | Jeannie Paris, R. Le Guellec, Michel Philippe, Christian Roghi, Anne Couturier |
---|---|
Rok vydání: | 1991 |
Předmět: |
Embryo
Nonmammalian Xenopus Molecular Sequence Data Restriction Mapping Kinesins Molecular cloning Aspergillus nidulans Sequence Homology Nucleic Acid Complementary DNA Schizosaccharomyces Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence Molecular Biology Adenosine Triphosphatases Cloning Base Sequence biology cDNA library Nucleic acid sequence Cell Biology biology.organism_classification Molecular biology Aspergillus Oocytes RNA Drosophila Research Article |
Zdroj: | Molecular and Cellular Biology. 11:3395-3398 |
ISSN: | 1098-5549 0270-7306 |
Popis: | By differential screening of a Xenopus egg cDNA library, we selected nine clones (Eg1 to Eg9) corresponding to mRNAs which are deadenylated and released from polysomes soon after fertilization. The sequence of one of these clones (Eg5) revealed that the corresponding protein has the characteristic features of a kinesin-related protein. More specifically, Eg5 was found to be nearly 30% identical to a kinesin-related protein encoded by bimc, a gene involved in nuclear division in Aspergillus nidulans. |
Databáze: | OpenAIRE |
Externí odkaz: |