PD-1 Involvement in Peripheral Blood CD8+ T Lymphocyte Dysfunction in Patients with Acute-on-chronic Liver Failure
| Autor: | Ninghui Zhao, Yidong Li, Jiefeng He, Jia Yao, Tian Liu, Yaqiu Ji, Xiaoshuang Zhou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Hepatology Glycogen biology business.industry CD69 T lymphocyte Immune function Glut1 chemistry.chemical_compound Endocrinology Immune system chemistry Internal medicine medicine biology.protein Glycolysis Tumor necrosis factor alpha GLUT1 Original Article Acute and chronic liver failure Programmed cell death 1 business CD8 |
| Zdroj: | Journal of Clinical and Translational Hepatology |
| ISSN: | 2310-8819 2225-0719 |
| Popis: | Background and Aims Programmed cell death-1 (PD-1) plays an important role in downregulating T lymphocytes but the mechanisms are still poorly understood. This study aimed to explore the role of PD-1 in CD8+ T lymphocyte dysfunction in hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF). Methods Thirty patients with HBV-ACLF and 30 healthy controls (HCs) were recruited. The differences in the numbers and functions of CD8+ T lymphocytes, PD-1 and glucose transporter-1 (Glut1) expression from the peripheral blood of patients with HBV-ACLF and HCs were analyzed. In vitro, the CD8+ T lymphocytes from HCs were cultured (HC group) and the CD8+ T lymphocytes from ACLF patients were cultured with PD-L1-IgG (ACLF+PD-1 group) or IgG (ACLF group). The numbers and functions of CD8+ T lymphocytes, PD-1 expression, glycogen uptake capacity, and Glut1, hexokinase-2 (HK2), and pyruvate kinase (PKM2) expression were analyzed among the HC group, ACLF group and ACLF+ PD-1group. Results The absolute numbers of CD8+ T lymphocytes in the peripheral blood from patients with HBV-ACLF were lower than in the HCs (p |
| Databáze: | OpenAIRE |
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