Lipofuscin Products, Lipid Peroxides and Aluminum Accumulation in Red Blood Cells of Hemodialyzed Patients
| Autor: | John Duett, Sushil K. Jain, M'Liss Sella, Kenneth Abreo |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Lipid Peroxides medicine.medical_specialty Erythrocytes Anemia Thiobarbituric acid Fluorescence Lipofuscin Nephropathy Pigment chemistry.chemical_compound Renal Dialysis Malondialdehyde Internal medicine medicine Humans business.industry Spectrophotometry Atomic Middle Aged medicine.disease Pathophysiology Red blood cell Spectrometry Fluorescence medicine.anatomical_structure Endocrinology chemistry Nephrology visual_art visual_art.visual_art_medium Kidney Failure Chronic Female lipids (amino acids peptides and proteins) Hemoglobin business Aluminum |
| Zdroj: | American Journal of Nephrology. 15:306-311 |
| ISSN: | 1421-9670 0250-8095 |
| DOI: | 10.1159/000168854 |
| Popis: | This study examines whether there is a relationship between aluminum overload and the accumulation of lipofuscin products (aging pigments) and lipid peroxides in red blood cells (RBC) of hemodialyzed patients. Lipid peroxides levels were assessed by the thiobarbituric acid reactivity; lipofuscin products were assessed by determining fluorescence in the lipid extracts at excitation 360 nm and emission 440 nm. Aluminum was measured by atomic absorption spectrophotometry. Controls were age-matched normal volunteers. Data show that there was a significant increase in the lipid peroxides and lipofuscin products in hemodialyzed patients compared with controls even after normalization with hemoglobin or phospholipids in RBC. Further, the increase in the lipid peroxides and lipofuscin products significantly correlated with the levels of aluminum accumulation in RBC of hemodialyzed patients. This study suggests that aluminum overload has a role in increased membrane peroxidation, which in turn can cause reduced RBC life span and contribute to anemia in chronic renal failure patients. |
| Databáze: | OpenAIRE |
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