Decreased Renal Expression of the Putative Calcium Oxalate Inhibitor Tamm-Horsfall Protein in the Ethylene Glycol Rat Model of Calcium Oxalate Urolithiasis
| Autor: | Lizhu Yang, Hue Lee C Kaung, Susan Ruth Marengo, Martin I. Resnick, Daniel H C Chen |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Kidney Tamm–Horsfall protein biology business.industry Urinary system Urology Calcium oxalate chemistry.chemical_element Calcium Oxalate chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Internal medicine biology.protein Medicine Osteopontin business Ethylene glycol |
| Zdroj: | The Journal of Urology. :2192-2197 |
| ISSN: | 0022-5347 |
| DOI: | 10.1097/00005392-200205000-00070 |
| Popis: | Purpose: Tamm-Horsfall protein is believed to inhibit calcium oxalate crystallization, aggregation or adhesion to the renal epithelium. We determined whether ethylene glycol induced urolithiasis changes the expression of renal and urinary Tamm-Horsfall protein. For comparison the expression of another calcium oxalate inhibitor, osteopontin, was also analyzed.Materials and Methods: Male rats were treated with 0.75% ethylene glycol plus an AIN-76 diet (Dyets, Bethlehem Pennsylvania) (ethylene glycol group) or standard rat chow and water (control group) for up to 8 weeks (6 per group for 8 weeks and 3 per group for 3 days to 6 weeks). Kidneys and urine (8 weeks only) were harvested and analyzed by Northern and Western blot analysis, and immunohistochemistry.Results: Tamm-Horsfall protein message and protein (membrane bound form) were decreased, while those of osteopontin were increased in the kidneys of rats treated with ethylene glycol for 8 weeks. As judged by immunochemistry Tamm-Horsfall protein and oste... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|