| Autor: |
Caride, Ariel, Jang, Jin Sung, Shi, Geng-Xian, Lenz, Sam, Zhong, Jian, Kim, Kwan Hyun, Allen, Mariet, Robertson, Keith D., Farrugia, Gianrico, Ordog, Tamas, Ertekin-Taner, Nilüfer, Lee, Jeong-Heon |
| Rok vydání: |
2023 |
| DOI: |
10.6084/m9.figshare.22620436 |
| Popis: |
Additional file 7: Figure S5. The quality and consistency of ChIP-seq libraries generated by the titration-based normalization approach. A-B: Correlation analysis between ChIP-seq datasets generated from different antibody titers. The Pearson’s correlation coefficient was visualized for the libraries generated from the conditions with the fixed amount of antibody (A) and normalized antibody amount to the optimal titer (B) in ChIP reactions. The library ID indicates DNAchrom (µg)-antibody titer in ChIP reactions. The libraries generated from 10 µg of DNAchrom was highlighted as green. C-D: Read intensities in promoter-associated peaks. Read count was visualized at peaks around TSSs for the libraries generated from fixed (C) or normalized (D) antibody amount. E: Fraction of reads in peaks (FRiP) is negatively correlated with antibody titer but improved when the antibody amount is normalized to the optimal titer. The score of FRiP was calculated by following the ENCODE guideline and visualized with DNAchrom and antibody titer. The libraries generated from 10 µg of DNAchrom was indicated as orange arrow. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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