Identification of growth regulators using cross-species network analysis in plants

Autor: Pasquale Luca Curci, Jie Zhang, Niklas Mähler, Carolin Seyfferth, Chanaka Mannapperuma, Tim Diels, Tom Van Hautegem, David Jonsen, Nathaniel Street, Torgeir R Hvidsten, Magnus Hertzberg, Ove Nilsson, Dirk Inzé, Hilde Nelissen, Klaas Vandepoele
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Plant Physiology
PLANT PHYSIOLOGY
ISSN: 0032-0889
1532-2548
Popis: Cross-species network analysis enables identification and validation of growth regulators in Arabidopsis. With the need to increase plant productivity, one of the challenges plant scientists are facing is to identify genes that play a role in beneficial plant traits. Moreover, even when such genes are found, it is generally not trivial to transfer this knowledge about gene function across species to identify functional orthologs. Here, we focused on the leaf to study plant growth. First, we built leaf growth transcriptional networks in Arabidopsis (Arabidopsis thaliana), maize (Zea mays), and aspen (Populus tremula). Next, known growth regulators, here defined as genes that when mutated or ectopically expressed alter plant growth, together with cross-species conserved networks, were used as guides to predict novel Arabidopsis growth regulators. Using an in-depth literature screening, 34 out of 100 top predicted growth regulators were confirmed to affect leaf phenotype when mutated or overexpressed and thus represent novel potential growth regulators. Globally, these growth regulators were involved in cell cycle, plant defense responses, gibberellin, auxin, and brassinosteroid signaling. Phenotypic characterization of loss-of-function lines confirmed two predicted growth regulators to be involved in leaf growth (NPF6.4 and LATE MERISTEM IDENTITY2). In conclusion, the presented network approach offers an integrative cross-species strategy to identify genes involved in plant growth and development.
Databáze: OpenAIRE
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