Bacterial Engulfment Mechanism Is Strongly Conserved in Evolution Between Earthworm and Human Immune Cells
| Autor: | Bohdana Kokhanyuk, Kornélia Bodó, György Sétáló Jr, Péter Németh, Péter Engelmann |
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| Rok vydání: | 2021 |
| Předmět: |
Cell type
Staphylococcus aureus THP-1 Cells Phagocytosis media_common.quotation_subject Immunology Endocytosis chemistry.chemical_compound coelomocytes Immune system evolution inhibitors Amantadine Escherichia coli Immunology and Allergy Animals Humans Oligochaeta Internalization Cytochalasin B Escherichia coli Infections Cytochalasin D media_common Original Research Messenger RNA Immunity Cellular Macrophages phagocytosis Cell Differentiation RC581-607 Staphylococcal Infections Biological Evolution Immunity Innate Cell biology chemistry Immunologic diseases. Allergy |
| Zdroj: | Frontiers in Immunology Frontiers in Immunology, Vol 12 (2021) |
| ISSN: | 1664-3224 |
| Popis: | Invertebrates, including earthworms, are applied to study the evolutionarily conserved cellular immune processes. Earthworm immunocytes (so-called coelomocytes) are functionally similar to vertebrate myeloid cells and form the first line of defense against invading pathogens. Hereby, we compared the engulfment mechanisms of THP-1 human monocytic cells, differentiated THP-1 (macrophage-like) cells, andEisenia andreicoelomocytes towardsEscherichia coliandStaphylococcus aureusbacteria applying various endocytosis inhibitors [amantadine, 5-(N-ethyl-N-isopropyl) amiloride, colchicine, cytochalasin B, cytochalasin D, methyl-ß-cyclodextrin, and nystatin]. Subsequently, we investigated the messenger RNA (mRNA) expressions of immune receptor-related molecules (TLR,MyD88,BPI) and the colocalization of lysosomes with engulfed bacteria following uptake inhibition in every cell type. Actin depolymerization by cytochalasin B and D has strongly inhibited the endocytosis of both bacterial strains in the studied cell types, suggesting the conserved role of actin-dependent phagocytosis. Decreased numbers of colocalized lysosomes/bacteria supported these findings. In THP-1 cellsTLRexpression was increased upon cytochalasin D pretreatment, while this inhibitor caused a droppedLBP/BPIexpression in differentiated THP-1 cells and coelomocytes. The obtained data reveal further insights into the evolution of phagocytes in eukaryotes. Earthworm and human phagocytes possess analogous mechanisms for bacterial internalization. |
| Databáze: | OpenAIRE |
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