Extraction of Aggrecan-Peptide from Cartilage by Tissue Autolysis
| Autor: | Anchalee Srichamroen, Takuo Nakano, Lech Ozimek |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Autolysis (biology)
animal structures Keratan sulfate Enzyme-Linked Immunosorbent Assay Uronic acid Patents as Topic Gel permeation chromatography chemistry.chemical_compound medicine Animals Aggrecans Chondroitin sulfate Aggrecan Chromatography biology Cartilage Chondroitin Sulfates General Medicine medicine.anatomical_structure chemistry Proteoglycan Biochemistry Keratan Sulfate biology.protein Autolysis Peptides |
| Zdroj: | Recent Patents on Food, Nutrition & Agriculture. 6:54-59 |
| ISSN: | 2212-7984 |
| Popis: | Aggrecan is a cartilage specific proteoglycan containing chondroitin sulfate (CS) and keratan sulfate (KS). CS is an acidic polysaccharide having wide range of applications in pharmaceutical, cosmetic, and food industries. CS is extracted from cartilage by tissue proteolysis with an exogenous proteinase or by activating endogenous proteinases (autolysis) to release aggrecan-peptides from the tissue. This review is focused on the latter technique. Bovine nasal and tracheal cartilages, and broiler chicken sternum cartilage have been used for autolysis studies. To extract aggrecan-peptide, cartilage tissues are cut into small pieces, and incubated in a monovalent or divalent salt solution (e.g., 0.1 M sodium or calcium acetate) at pH 4.5 and 37 °C for 7 - 24 h. Most (~80% or more) of total tissue uronic acid, a constituent sugar of aggrecan, is extracted and released into the salt solution during incubation. Reextraction of the tissue residue results in release of a small amount of uronic acid. Aggrecan-peptides purified using anion exchange chromatography are large compounds containing CS and KS. On gel chromatography, they are excluded from the column of Sephacryl S-300. Chemical composition analysis demonstrated that aggrecan-peptides from either bovine or chicken cartilage contain >90% CS with small amount (< 10%) of either KS or peptide. Patent information included production of aggrecan-peptide substantially free of DNA. The bovine aggrecan-peptide prepared by tissue autolysis has been used as a plate coating antigen in enzyme- linked immunosorbent assay (ELISA) to determine KS. |
| Databáze: | OpenAIRE |
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