High Fat Diet Increases Circulating Endocannabinoids Accompanied by Increased Synthesis Enzymes in Adipose Tissue
| Autor: | Eline N. Kuipers, Vasudev Kantae, Boukje C. Eveleens Maarse, Susan M. van den Berg, Robin van Eenige, Kimberly J. Nahon, Anne Reifel-Miller, Tamer Coskun, Menno P. J. de Winther, Esther Lutgens, Sander Kooijman, Amy C. Harms, Thomas Hankemeier, Mario van der Stelt, Patrick C. N. Rensen, Mariëtte R. Boon |
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| Přispěvatelé: | Experimental Immunology, Medical Biochemistry, ACS - Atherosclerosis & ischemic syndromes, AII - Inflammatory diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Calorie diet-induced obesity Physiology Adipose tissue White adipose tissue NAPE-PLD lcsh:Physiology chemistry.chemical_compound Downregulation and upregulation white adipose tissue Internal medicine Physiology (medical) Brown adipose tissue medicine endocannabinoids Original Research lcsh:QP1-981 Chemistry brown adipose tissue Anandamide medicine.disease Obesity Endocannabinoid system medicine.anatomical_structure Endocrinology lipids (amino acids peptides and proteins) |
| Zdroj: | Frontiers in physiology, 10(JAN):1913. Frontiers Media S.A. Frontiers in Physiology Frontiers in Physiology, 9, 1913 Frontiers in Physiology, 9 Frontiers in Physiology, Vol 9 (2019) |
| ISSN: | 1664-042X |
| DOI: | 10.3389/fphys.2018.01913 |
| Popis: | The endocannabinoid system (ECS) controls energy balance by regulating both energy intake and energy expenditure. Endocannabinoid levels are elevated in obesity suggesting a potential causal relationship. This study aimed to elucidate the rate of dysregulation of the ECS, and the metabolic organs involved, in diet-induced obesity. Eight groups of age-matched male C57Bl/6J mice were randomized to receive a chow diet (control) or receive a high fat diet (HFD, 45% of calories derived from fat) ranging from 1 day up to 18 weeks before euthanasia. Plasma levels of the endocannabinoids 2-arachidonoylglycerol (2-AG) and anandamide (N-arachidonoylethanolamine, AEA), and related N-acylethanolamines, were quantified by UPLC-MS/MS and gene expression of components of the ECS was determined in liver, muscle, white adipose tissue (WAT) and brown adipose tissue (BAT) during the course of diet-induced obesity development. HFD feeding gradually increased 2-AG (+132% within 4 weeks, P < 0.05), accompanied by upregulated expression of its synthesizing enzymes Daglα and β in WAT and BAT. HFD also rapidly increased AEA (+81% within 1 week, P < 0.01), accompanied by increased expression of its synthesizing enzyme Nape-pld, specifically in BAT. Interestingly, Nape-pld expression in BAT correlated with plasma AEA levels (R2 = 0.171, β = 0.276, P < 0.001). We conclude that a HFD rapidly activates adipose tissue depots to increase the synthesis pathways of endocannabinoids that may aggravate the development of HFD-induced obesity.. |
| Databáze: | OpenAIRE |
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