Bioresponsive peptide-polysaccharide nanogels — A versatile delivery system to augment the utility of bioactive cargo
| Autor: | Andrew W. Simonson, Kenneth C. Keiler, Scott H. Medina, Atip Lawanprasert, Tyler D. P. Goralski |
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| Rok vydání: | 2019 |
| Předmět: |
Endosome
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Peptide Context (language use) 02 engineering and technology Article 03 medical and health sciences Drug Delivery Systems Human Umbilical Vein Endothelial Cells Peptide amphiphile Humans Nanotechnology Polylysine General Materials Science Hyaluronic Acid 030304 developmental biology chemistry.chemical_classification Drug Carriers 0303 health sciences Chemistry 021001 nanoscience & nanotechnology Small molecule Nanostructures Membrane A549 Cells Drug delivery Biophysics Molecular Medicine 0210 nano-technology Gels Nanogel |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 17:391-400 |
| ISSN: | 1549-9634 |
| Popis: | We report the design, synthesis and efficacy of a new class of gel-like nano-carrier, or 'nanogel', prepared via templated electrostatic assembly of anionic hyaluronic acid (HA) polysaccharides with the cationic peptide amphiphile poly-L-lysine (PLL). Small molecules and proteins present during nanogel assembly become directly encapsulated within the carrier and are precisely released by tuning the nanogel HA:PLL ratio to control particle swelling. Remarkably, nanogels exhibit versatile and complimentary mechanisms of cargo delivery depending on the biologic context. For example, in mammalian cells, nanogels are rapidly internalized and escape the endosome to both deliver membrane-impermeable protein cargo into the cytoplasm and improve chemotherapeutic potency in drug resistant cancer cells. In bacteria, nanogels permeabilize microbial membranes to sensitize bacterial pathogens to the action of a loaded antibiotic. Thus, peptide nanogels represent a versatile, readily scalable and bio-responsive carrier capable of augmenting and enhancing the utility of a broad range of biomolecular cargoes. |
| Databáze: | OpenAIRE |
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