Differential roles of SS18–SSX fusion gene and insulin-like growth factor-1 receptor in synovial sarcoma cell growth
| Autor: | Maria Törnkvist, Leonard Girnita, Natalia Natalishvili, Magnus Axelson, Ada Girnita, Yuntao Xie, Padraig D'Arcy, Bertha Brodin |
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| Rok vydání: | 2008 |
| Předmět: |
Oncogene Proteins
Fusion DNA synthesis Cell growth medicine.medical_treatment Biophysics Apoptosis Cell Biology Biology medicine.disease Biochemistry Synovial sarcoma Receptor IGF Type 1 Fusion gene Sarcoma Synovial Insulin-like growth factor Cyclin D1 Cell Line Tumor medicine Cancer research Humans Viability assay Molecular Biology Cell Proliferation Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 368:793-800 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2008.01.162 |
| Popis: | Recently we demonstrated that the synovial sarcoma specific fusion gene SS18-SSX is crucial for cyclin D1 expression and is linked to cell proliferation. In this report we explore the role of SS18-SSX and IGF-1R for their potential functions in cellular proliferation and survival in cultured synovial sarcoma cells. We found that targeting of SS18-SSX mRNA by antisense oligonucleotide treatment drastically and rapidly decreased cell proliferation but caused only a slight increase of apoptosis. The synovial sarcoma cells were confirmed to express IGF-1R, and treatment with an IGF-1R inhibitor resulted in substantially reduced cell viability by inducing apoptosis in these cells. Conversely, inhibition of the IGF-1R resulted only in a slight to moderate decrease in DNA synthesis. In conclusion, SS18-SSX and IGF-1R seem to play important but different roles in maintaining malignant growth of synovial sarcoma cells. Whereas SS18-SSX maintains cyclin D1 and cell proliferation, IGF-1R protects from apoptosis. |
| Databáze: | OpenAIRE |
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