Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection

Autor:	Boqing Li, Lei Zhang, Peiyou Gong, Ping Yang, Tianci Yang, Junjie Liu, Xiuzhi Zhou
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	0301 basic medicine MAPK/ERK pathway Messenger RNA biology PITX2 Article Subject QD415-436 Helicobacter pylori biology.organism_classification Biochemistry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Mitogen-activated protein kinase microRNA Cancer research biology.protein Signal transduction Gene
Zdroj:	Biochemistry Research International, Vol 2020 (2020)
ISSN:	2090-2247
DOI:	10.1155/2020/6285987
Popis:	Background. Helicobacter pylori (H. pylori) is a common human pathogen, which is closely correlated with gastric cancer (GC). However, the mechanism of H. pylori-related GC has not been elucidated. This study aimed to explore the role of H. pylori infection in GC and find biomarkers for early diagnosis of H. pylori-related GC. Methods. We identified differentially expressed microRNAs (DEMs) and genes (DEGs) from the Gene Expression Omnibus (GEO) dataset, constructed microRNA-(miRNA-)mRNA expression networks, analyzed the function and signal pathway of cross-genes, analyzed the relations between cross-genes and GC prognosis with the Cancer Genome Atlas (TCGA) data, and verified the expression of cross-genes in patients with H. pylori infection. Results. 22 DEMs and 68 DEGs were identified in GSE197694 and GSE27411 dataset. 16 miRNAs and 509 genes were involved in the expression network, while the cross-genes of the network were mainly enriched in MAP kinase (MAPK) signaling pathway and TGF-beta signaling pathway. Patients with higher expression of hsa-miR-196b-3p, CALML4, or SMAD6 or lower expression of PITX2 or TGFB2 had better outcomes than those with lower expression of hsa-miR-196b-3p, CALML4, or SMAD6 or higher expression of PITX2 or TGFB2 (P<0.05). Patients with H. pylori infection had a higher expression of hsa-miR-196b-3p and CALML4 than those without H. pylori infection (P<0.05). Conclusion. The study of miRNA-mRNA expression network would provide molecular support for early diagnosis and treatment of H. pylori-related GC.
Databáze:	OpenAIRE
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